Studies on the initiation of protein synthesis in mouse myeloma tumors
dc.contributor.author | Jones, George H. | en_US |
dc.date.accessioned | 2006-04-07T16:41:22Z | |
dc.date.available | 2006-04-07T16:41:22Z | |
dc.date.issued | 1975 | en_US |
dc.identifier.citation | Jones, George H. (1975)."Studies on the initiation of protein synthesis in mouse myeloma tumors." Archives of Biochemistry and Biophysics 170(): 409-416. <http://hdl.handle.net/2027.42/22179> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DW2FWY-WF/2/e507869783d112dcde243c352eb6cfce | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22179 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1190773&dopt=citation | en_US |
dc.description.abstract | 35S- and 3H-labeled short, nascent peptides have been extracted from mouse myeloma ribosomes after incubation of myeloma fragments with [35S]methionine and [3H]amino acids. Edman analysis of these peptides reveals that most of the methionine is present at the N terminus but that other N-terminal amino acids are also present. Light chains synthesized by the RPC-20 tumor fragments were purified from ribosomes, cell sap (released light chain) and the incubation medium (secreted light chain). Ribosome-bound light chains were found to possess some N-terminal methionine whereas released and secreted light chains did not. Since methionine is not the N-terminal amino acid of light (L) chains purified from the urine of tumor-bearing mice, the results indicate that methionine initiates L chain biosynthesis in the myeloma.Total nascent 35S-labeled peptides were extracted from myeloma ribosomes and fractionated on Sephadex G-50. Edman analysis of Chromatographic fractions of varying sizes indicated that the percentage of N-terminal methionine decreased with increasing chain length. This behavior is expected if methionine serves as an initiator amino acid but is removed before completion of the polypeptide chain on the ribosome. | en_US |
dc.format.extent | 685094 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Studies on the initiation of protein synthesis in mouse myeloma tumors | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Zoology, University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.identifier.pmid | 1190773 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22179/1/0000610.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(75)90136-8 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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