Genetic characterization of a bacterial locus involved in the activity of the N function of phage [lambda]
dc.contributor.author | Friedman, David I. | en_US |
dc.contributor.author | Baron, L. S. | en_US |
dc.date.accessioned | 2006-04-07T16:48:00Z | |
dc.date.available | 2006-04-07T16:48:00Z | |
dc.date.issued | 1974-03 | en_US |
dc.identifier.citation | Friedman, David I., Baron, L. S. (1974/03)."Genetic characterization of a bacterial locus involved in the activity of the N function of phage [lambda]." Virology 58(1): 141-148. <http://hdl.handle.net/2027.42/22397> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WXR-4BNMN7S-HH/2/bd386ccf7eafbffd7ae59fcd37904425 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22397 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=4595374&dopt=citation | en_US |
dc.description.abstract | We report the genetic mapping of a locus of the Escherichia coli chromosome involved in the expression of the N gene function of phage [lambda]. This phage specified function regulates the subsequent transcription of most of the [lambda] genome. The bacterial locus involved in N expression, called nus for N utilization substance, maps between aspB at minute 62 and argG at minute 61 of the E. coli chromosome.Two different bacterial variants in which [lambda] N function is not active have been used in mapping the nus locus, a mutant of E. coli K12, Nus, and a hybrid bacterium formed by genetic transfer between E. coli and S. typhosa. Although these two bacterial variants exhibit slightly different phenotypes, chromosome transfer studies demonstrate that the same genetic region is involved in the observed N-ineffective phenotype.Dominance studies show that in the case of the Nus mutant, the nus+ allele is dominant. This suggests that the nus+ allele is responsible for the expression of a function necessary for N product activity. In the case of transfer of the nus region from a Nus mutant to an E. coli-S. typhosa hybrid, the resulting hybrid assumes the phenotype of the Nus mutant. Genetic studies using P1 transduction demonstrate that the same genetic region is involved in the N-ineffective phenotype of the two bacterial variants. | en_US |
dc.format.extent | 782570 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Genetic characterization of a bacterial locus involved in the activity of the N function of phage [lambda] | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Bacterial Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012, USA; Department of Microbiology, Medical School, The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Department of Bacterial Immunology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012, USA; Department of Microbiology, Medical School, The University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.identifier.pmid | 4595374 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22397/1/0000847.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0042-6822(74)90149-4 | en_US |
dc.identifier.source | Virology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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