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Brief communication: Technical modifications of the human agarose microdroplet leukocyte migration inhibition assay
dc.contributor.authorWeese, James L.en_US
dc.contributor.authorMcCoy, James L.en_US
dc.contributor.authorDean, Jack H.en_US
dc.contributor.authorOrtaldo, John R.en_US
dc.contributor.authorBurk, Kathleen R.en_US
dc.contributor.authorHerberman, Ronald B.en_US
dc.date.accessioned2006-04-07T16:57:32Z
dc.date.available2006-04-07T16:57:32Z
dc.date.issued1978-12en_US
dc.identifier.citationWeese, James L., McCoy, James L., Dean, Jack H., Ortaldo, John R., Burk, Kathleen R., Herberman, Ronald B. (1978/12)."Brief communication: Technical modifications of the human agarose microdroplet leukocyte migration inhibition assay." Journal of Immunological Methods 24(3-4): 363-370. <http://hdl.handle.net/2027.42/22477>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T2Y-476KY7V-2RR/2/18d2b56e89a073cf530b53ba1699e99fen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/22477
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=722101&dopt=citationen_US
dc.description.abstractDirect leukocyte migration inhibition assays using the capillary tube technique can be used to demonstrate cell-mediated immunity in vitro. Unfortunately, the cumbersome nature of this technique makes it time consuming and difficult to perform. Similar results have been obtained using the direct agarose microdroplet leukocyte migration inhibition assay. In this paper, modifications of the agarose technique are outlined which insure standardization of droplets and ease of performance of the assay. Additionally a technique is described to reduce the time required for calculation of results.en_US
dc.format.extent377259 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleBrief communication: Technical modifications of the human agarose microdroplet leukocyte migration inhibition assayen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumLaboratory of Immunodiagnosis, National Cancer Institute, Bethesda, MD 20014, USA: Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48104, USAen_US
dc.contributor.affiliationotherLaboratory of Immunodiagnosis, National Cancer Institute, Bethesda, MD 20014, USAen_US
dc.contributor.affiliationotherDepartment of Immunology, Litton Bionetics Research Laboratories, Kensington, MD 20975, USAen_US
dc.contributor.affiliationotherLaboratory of Immunodiagnosis, National Cancer Institute, Bethesda, MD 20014, USAen_US
dc.contributor.affiliationotherLaboratory of Immunodiagnosis, National Cancer Institute, Bethesda, MD 20014, USAen_US
dc.contributor.affiliationotherLaboratory of Immunodiagnosis, National Cancer Institute, Bethesda, MD 20014, USAen_US
dc.identifier.pmid722101en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/22477/1/0000018.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0022-1759(78)90139-4en_US
dc.identifier.sourceJournal of Immunological Methodsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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