L-threonine dehydrogenase of Escherichia coli K-12
dc.contributor.author | Boylan, Sharon A. | en_US |
dc.contributor.author | Dekker, Eugene E. | en_US |
dc.date.accessioned | 2006-04-07T16:57:54Z | |
dc.date.available | 2006-04-07T16:57:54Z | |
dc.date.issued | 1978-11-14 | en_US |
dc.identifier.citation | Boylan, Sharon A., Dekker, Eugene E. (1978/11/14)."L-threonine dehydrogenase of Escherichia coli K-12." Biochemical and Biophysical Research Communications 85(1): 190-197. <http://hdl.handle.net/2027.42/22489> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4G0VNMJ-TB/2/76f4b5a192a7c483daef324a6dee6774 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22489 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=33672&dopt=citation | en_US |
dc.description.abstract | SummaryA rapid method for purifying L-threonine dehydrogenase from Escherichia coli K-12 cells, grown on a medium containing L-threonine as carbon source, has been developed. The procedure consists of three fractionation steps of which the last is adsorption and elution of the enzyme from a column of Blue dextran-Sepharose. Homogeneous and stable samples of the dehydrogenase are obtained. L-Threonine dehydrogenase of E. coli has an average molecular weight of 140,000 and consists of 4 identical or nearly identical subunits. Km values for L-threonine and NAD[circle plus operator] are 1.4 mM and 0.19 mM, respectively; several substrate and coenzyme analogs are also active. The pH optimum is 10.3 and Mn++ stimulates dehydrogenase activity. Other properties of the pure enzyme have been established. | en_US |
dc.format.extent | 883073 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | L-threonine dehydrogenase of Escherichia coli K-12 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The Department of Biological Chemistry, The University of Michigan Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | The Department of Biological Chemistry, The University of Michigan Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 33672 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22489/1/0000030.pdf | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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