Post-thaw suspension of red cells cryopreserved with hydroxyethyl starch
dc.contributor.author | Allen, Edward D. | en_US |
dc.contributor.author | Weatherbee, Lee | en_US |
dc.contributor.author | Permoad, P. A. | en_US |
dc.date.accessioned | 2006-04-07T16:59:58Z | |
dc.date.available | 2006-04-07T16:59:58Z | |
dc.date.issued | 1978-08 | en_US |
dc.identifier.citation | Allen, E. D., Weatherbee, L., Permoad, P. A. (1978/08)."Post-thaw suspension of red cells cryopreserved with hydroxyethyl starch." Cryobiology 15(4): 375-381. <http://hdl.handle.net/2027.42/22557> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WD5-4F1HR49-164/2/0912b80722ed4e4adf2ed74e68fe1181 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22557 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=81119&dopt=citation | en_US |
dc.description.abstract | Post-thaw suspension of red cells cryopreserved with hydroxyethyl starch (HES) gives rise to an increase in the quantity of supernatant hemoglobin and a reduction in cell recovery. This occurs regardless of the suspension media used but may be delayed by some solutions. Extended suspension with most solutions results in loss of intracellular contents from a number of cells. The resulting ghosts account for 12-14% of the total cell population. In the presence of 14% HES, deposits on the surface of damaged cells indicate that cellular contents are adherent to the cell membrane and therefore do not contribute to the free hemoglobin in the external solution. The result is a misleading high cell recovery value and suggests that this test does not accurately describe true cell damage in the presence of HES. Since the saline stability of suspended cells changes very little from that prior to suspension (at thaw), its value is a more accurate assessment of cell damage. | en_US |
dc.format.extent | 4678635 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Post-thaw suspension of red cells cryopreserved with hydroxyethyl starch | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Medical Research, Veterans Administration Hospital and University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A. | en_US |
dc.contributor.affiliationum | Medical Research, Veterans Administration Hospital and University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A. | en_US |
dc.contributor.affiliationum | Medical Research, Veterans Administration Hospital and University of Michigan Medical Center, Ann Arbor, Michigan 48105, U.S.A. | en_US |
dc.identifier.pmid | 81119 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22557/1/0000102.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0011-2240(78)90055-X | en_US |
dc.identifier.source | Cryobiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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