A rapid sensitive assay for phosphatidate phosphohydrolase
dc.contributor.author | Bleasdale, John E. | en_US |
dc.contributor.author | Davis, Cinda-Sue | en_US |
dc.contributor.author | Hajra, Amiya K. | en_US |
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.date.accessioned | 2006-04-07T17:00:55Z | |
dc.date.available | 2006-04-07T17:00:55Z | |
dc.date.issued | 1978-06-15 | en_US |
dc.identifier.citation | Bleasdale, J. E., Davis, C. S., Hajra, A. K., Agranoff, B. W. (1978/06/15)."A rapid sensitive assay for phosphatidate phosphohydrolase." Analytical Biochemistry 87(1): 19-27. <http://hdl.handle.net/2027.42/22588> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DX49V9-X7/2/2fcca7f113938e075062d4e0525dfc41 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22588 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=27995&dopt=citation | en_US |
dc.description.abstract | For a purified preparation of the soluble form of phosphatidate phosphohydrolase (EC 3.1.3.4) from guinea pig cerebral cortex, 1-O-alkyl-rac-glycerol 3-phosphate was found to be accepted as a substrate. This substrate analog was tritium-labeled in order to serve in a rapid sensitive assay for the enzyme, in which labeled 1-alkyl glycerol is released. Heat denaturation and enzyme activity dependence on pH indicated that 1-O-alkyl-rac-glycerol 3-phosphate phosphohydrolase and phosphatidate phosphohydrolase activities in the preparation are attributable to the same enzyme. 1-O-Alkyl-rac-glycerol 3-phosphate was hydrolyzed with a Vmax of 1.7 nmol min-1 mg-1 of protein and a Km of 270 [mu]. | en_US |
dc.format.extent | 530210 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A rapid sensitive assay for phosphatidate phosphohydrolase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA; Neuroscience Laboratory, Mental Health Research Institute, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA; Neuroscience Laboratory, Mental Health Research Institute, The University of Michigan, Ann Arbor, Michigan 48109, USA. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA; Neuroscience Laboratory, Mental Health Research Institute, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA; Neuroscience Laboratory, Mental Health Research Institute, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 27995 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22588/1/0000136.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(78)90564-X | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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