Use of salicylic acid to measure the apparent intracellular pH in the ehrlich ascites-tumor cell and Escherichia coli
dc.contributor.author | Garcia-Sancho, Javier | en_US |
dc.contributor.author | Sanchez, Ana | en_US |
dc.date.accessioned | 2006-04-07T17:01:21Z | |
dc.date.available | 2006-04-07T17:01:21Z | |
dc.date.issued | 1978-05-04 | en_US |
dc.identifier.citation | Garcia-Sancho, Javier, Sanchez, Ana (1978/05/04)."Use of salicylic acid to measure the apparent intracellular pH in the ehrlich ascites-tumor cell and Escherichia coli." Biochimica et Biophysica Acta (BBA) - Biomembranes 509(1): 148-158. <http://hdl.handle.net/2027.42/22602> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1T-47T85TG-1T/2/22b768aa79a84881e5c1cd14a13110b0 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22602 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=25670&dopt=citation | en_US |
dc.description.abstract | The distribution of salicylic acid between the intracellular and extracellular phases has been used to estimate the intracellular pH in the Ehrlich cell and Escherichia coli. The validity of the method was established by: (i) comparison of the results obtained with salicylic acid with those obtained with 5,5-dimethyloxazolidine-2,4-dione; (ii) by following changes of the apparent intracellular pH under circumstances in which such changes are predictable, e.g., the addition of weak acids or proton conductors to the incubation medium during incubation at acidic pH; (iii) by comparison of the apparent intracellular pH changes with the uptake of H+ by the cells estimated from the changes of the medium pH. Optimal results are obtained with this indicator when the extracellular pH is below 5.5, because in this case the indicator is to a sufficient extent in its penetrating form, so that its movement can reflect intracellular pH changes occurring in less than 30 s. When the intracellular pH falls below 5.2 measurable binding of salicylic acid to the intracellular material of the Ehrlich cell takes place, but above this pH no binding has been found.The Ehrlich cell and cells of Escherichia coli behaved similarly under various experimental circumstances tested, but striking differences were found in the inherent permeability of the membrane to H+ and in the changes in this parameter by lowering the temperature to 2[deg]C. | en_US |
dc.format.extent | 616521 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Use of salicylic acid to measure the apparent intracellular pH in the ehrlich ascites-tumor cell and Escherichia coli | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departamento de Bioquímica, Facultad de Medicina, Santander, Spain; Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Departamento de Bioquímica, Facultad de Medicina, Santander, Spain; Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.identifier.pmid | 25670 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22602/1/0000151.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2736(78)90015-9 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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