The effects of dibutyryl cyclic adenosine 3': 5'-monophosphate on concanavalin a-stimulated sterol and fatty acid synthesis in mouse spleen lymphocytes
dc.contributor.author | Respess, James G. | en_US |
dc.contributor.author | Stubbs, John D. | en_US |
dc.contributor.author | Chambers, Donald A. | en_US |
dc.date.accessioned | 2006-04-07T17:01:41Z | |
dc.date.available | 2006-04-07T17:01:41Z | |
dc.date.issued | 1978-04-28 | en_US |
dc.identifier.citation | Respess, James G., Stubbs, John D., Chambers, Donald A. (1978/04/28)."The effects of dibutyryl cyclic adenosine 3': 5'-monophosphate on concanavalin a-stimulated sterol and fatty acid synthesis in mouse spleen lymphocytes." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 529(1): 38-43. <http://hdl.handle.net/2027.42/22613> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1X-47F72KR-V0/2/08d06f5b54f0d77f82a7f767c239c5c9 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22613 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=205260&dopt=citation | en_US |
dc.description.abstract | Substantial increases in both 3[beta]-OH sterol and fatty acid synthesis were observed after concanavalin A addition to mouse spleen lymphocytes cultured in serum-free media. The rate of sterol synthesis increased linearly up to 60 h. The rate of fatty acid synthesis increased up to 20 h, reaching a plateau in synthetic activity which was the maintained. CO2 production from acetate was slightly stimulated by concanavalin A. In contrast to sterol and fatty acid synthesis, the rate of CO2 production in both mitogen-stimulated and resting cultures declined with time. Dibutyryl cyclic AMP had a strong inhibitory effect on concanavalin A-stimulated sterol and fatty acid synthesis from acetate, but only a slight effect on CO2 production. Delayed addition of dibutyryl cyclic AMP resulted in reduced inhibition. The data suggest a sequence of initiation for fatty acid and sterol synthesis prior to DNA synthesis and a possible regulatory role of cyclic AMP in this initiation. The results support the hypothesis that lymphocyte activation is sequential within the spleen cell population and is accompanied by fatty acid and sterol synthesis. | en_US |
dc.format.extent | 437973 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | The effects of dibutyryl cyclic adenosine 3': 5'-monophosphate on concanavalin a-stimulated sterol and fatty acid synthesis in mouse spleen lymphocytes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Dermatology and Biological Chemistry, University of Michigan Medical School, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Cell and Molecular Biology, San Francisco State University, San Francisco, Calif. 94132, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Cell and Molecular Biology, San Francisco State University, San Francisco, Calif. 94132, U.S.A. | en_US |
dc.identifier.pmid | 205260 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22613/1/0000163.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2760(78)90101-7 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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