2-acetamido-2-deoxy-[alpha]-D-galactosidases of clostridium perfringens. separation and characterization of an exoglycosidase and an oligosaccharidase
dc.contributor.author | Bell, William C. | en_US |
dc.contributor.author | Pomato, Nicholas | en_US |
dc.contributor.author | Aminoff, David | en_US |
dc.date.accessioned | 2006-04-07T17:02:53Z | |
dc.date.available | 2006-04-07T17:02:53Z | |
dc.date.issued | 1978-03 | en_US |
dc.identifier.citation | Bell, William C., Pomato, Nicholas, Aminoff, David (1978/03)."2-acetamido-2-deoxy-[alpha]-D-galactosidases of clostridium perfringens. separation and characterization of an exoglycosidase and an oligosaccharidase." Carbohydrate Research 61(1): 447-455. <http://hdl.handle.net/2027.42/22650> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TFF-42HG8VW-5P/2/c034f686242b05398f3a5fa77fd3b98e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22650 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=206361&dopt=citation | en_US |
dc.description.abstract | Two distinct 2-acetamido-2-deoxy-[alpha]-D-galactosidases have been separated from filtrates of cultured Clostridium perfringens by electrophoresis in 6.5% poly(acryl-amide) gels. One of the enzymes had a mobility of 0.32-0.36 (relative to Bromophenol Blue) and was identified as the exoglycosidase, 2-acetamido-2-deoxy-[alpha]-D-galactosidase. It appears to be the same enzyme as that reported in 1972 by McGuire et al. The second of the two ezymes, having a relative mobility of 0.42-0.46, corresponds to the oligosaccharidase reported in 1972 by Huang and Aminoff. The A-specificities of human type-A erythrocytes and of water-soluble glycoproteins having A-activity are both destroyed by incubation with the 2-acetamido-2-deoxy-[alpha]-D-galactosidase, but not on incubation with the oligosaccharidase. A concomitant rise in blood-group O(H) activity, as indicated by the use of a lectin from Ulex europeus, occurred in the A-erythrocytes treated with the exoglycosidase 2-acetamido-2-deoxy-[alpha]-D-galactosidase. | en_US |
dc.format.extent | 1408523 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | 2-acetamido-2-deoxy-[alpha]-D-galactosidases of clostridium perfringens. separation and characterization of an exoglycosidase and an oligosaccharidase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine (Simpson Memorial Institutes) and Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine (Simpson Memorial Institutes) and Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine (Simpson Memorial Institutes) and Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 U.S.A. | en_US |
dc.identifier.pmid | 206361 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22650/1/0000201.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0008-6215(00)84504-8 | en_US |
dc.identifier.source | Carbohydrate Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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