LH biosynthesis and secretion in rat anterior pituitary cell cultures: Stimulation of LH glycosylation and secretion by GNRH and an agonistic analogue and blockade by an antagonistic analogue
dc.contributor.author | Azhar, Salman | en_US |
dc.contributor.author | Reel, J. R. | en_US |
dc.contributor.author | Pastushok, C. A. | en_US |
dc.contributor.author | Menon, K. M. J. | en_US |
dc.date.accessioned | 2006-04-07T17:03:06Z | |
dc.date.available | 2006-04-07T17:03:06Z | |
dc.date.issued | 1978-02-14 | en_US |
dc.identifier.citation | Azhar, S., Reel, J. R., Pastushok, C. A., Menon, K. M. J. (1978/02/14)."LH biosynthesis and secretion in rat anterior pituitary cell cultures: Stimulation of LH glycosylation and secretion by GNRH and an agonistic analogue and blockade by an antagonistic analogue." Biochemical and Biophysical Research Communications 80(3): 659-666. <http://hdl.handle.net/2027.42/22657> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4F03B28-56/2/96cda426aee1a4237f33278162f718c4 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22657 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=343785&dopt=citation | en_US |
dc.description.abstract | In rat anterior pituitary cell cultures GnRH (1nM) stimulated a progressive increase in LH release into the medium from 1 to 8 h of incubation, while cellular LH showed a corresponding decrease. GnRH (1nM) neither modified the uptake nor the incorporation of [3H]-glucosamine and [3H]-proline into total protein. The incorporation of [3H]-proline into cellular LH also was unaffected by GnRH. In contrast, GnRH stimulated a 3 to 4-fold increase in [3H]-glucosamine incorporation into cellular LH. The agonistic analogue, [des GlyNH210]-LHRH ethylamide, mimicked the GnRH effects and was 5 to 6 times more potent than GnRH. The antagonistic analogue, [D-Phe2, D-Phe6]-LHRH blocked the GnRH-stimulated effects. These results suggest that GnRH and agonistic analogues may preferentially regulate turnover or synthesis of the carbohydrate moiety of LH. | en_US |
dc.format.extent | 481765 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | LH biosynthesis and secretion in rat anterior pituitary cell cultures: Stimulation of LH glycosylation and secretion by GNRH and an agonistic analogue and blockade by an antagonistic analogue | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Endocrine Laboratory, Departments of Obstetrics and Gynecology and Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Endocrine Laboratory, Departments of Obstetrics and Gynecology and Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Department of Pharmacology, Warner-Lambert/Parke-Davis Pharmaceutical Research Division, Ann Arbor, Michigan 48106, USA | en_US |
dc.contributor.affiliationother | Department of Pharmacology, Warner-Lambert/Parke-Davis Pharmaceutical Research Division, Ann Arbor, Michigan 48106, USA | en_US |
dc.identifier.pmid | 343785 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22657/1/0000208.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(78)91619-4 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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