Replication in situ and DNA encapsulation following induction of an excision-defective lysogen of Salmonella bacteriophage P22
dc.contributor.author | Weaver, Steven | en_US |
dc.contributor.author | Levine, Myron | en_US |
dc.date.accessioned | 2006-04-07T17:03:44Z | |
dc.date.available | 2006-04-07T17:03:44Z | |
dc.date.issued | 1978-01-25 | en_US |
dc.identifier.citation | Weaver, Steven, Levine, Myron (1978/01/25)."Replication in situ and DNA encapsulation following induction of an excision-defective lysogen of Salmonella bacteriophage P22." Journal of Molecular Biology 118(3): 389-411. <http://hdl.handle.net/2027.42/22678> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WK7-4DM28YR-4X/2/abd6c14e06817fe0e7decd4911a9742e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22678 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=344889&dopt=citation | en_US |
dc.description.abstract | The induction of an excision-defective bacteriophage P22 lysogen results in the production of particles which carry a DNA molecule of normal length within a normal capsid, but which are nonetheless defective. The DNA content of these particles was characterized physically by a restriction enzyme analysis, and genetically by two marker rescue techniques. The particles carry DNA corresponding to one side of the prophage map as well as additional DNA, apparently derived from the host chromosome to one side of the prophage insertion site. Normally, mature P22 DNA molecules are derived from a concatemer by sequential cleavage of adjacent headful lengths, beginning at a genetically unique site, the encapsulation origin (Tye et al., 1974). The defective particles appear to contain DNA matured by the same sequential mechanisms, operating on the integrated prophage and neighboring bacterial chromosome, rather than on the normal concatemeric substrate. Both the initiation and directional specificities of normal maturation are maintained during the maturation of defective particle DNA. Sequential cleavage begins within the prophage at the encapsulation origin, a site near gene 3, and proceeds into the host chromosome on the proC side of the prophage. The initiation specificity of DNA encapsulation seems to reside in the morphogenetic machinery, rather than in the mechanism of DNA replication. Replication of an induced excision-defective prophage takes place in situ on the host chromosome, apparently without disruption of the linear integrity of the prophage. Further, the entire prophage, as well as adjacent bacterial DNA, is replicated, even though only a portion of this DNA is destined to be encapsulated. | en_US |
dc.format.extent | 2049370 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Replication in situ and DNA encapsulation following induction of an excision-defective lysogen of Salmonella bacteriophage P22 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.identifier.pmid | 344889 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22678/1/0000231.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-2836(78)90235-8 | en_US |
dc.identifier.source | Journal of Molecular Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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