Plasma ADP levels: Direct determination with luciferase luminescence using a biometer
dc.contributor.author | Jabs, Clarence M. | en_US |
dc.contributor.author | Ferrell, William J. | en_US |
dc.contributor.author | Robb, Herbert J. | en_US |
dc.date.accessioned | 2006-04-07T17:05:05Z | |
dc.date.available | 2006-04-07T17:05:05Z | |
dc.date.issued | 1978 | en_US |
dc.identifier.citation | Jabs, Clarence M., Ferrell, William J., Robb, Herbert J. (1978)."Plasma ADP levels: Direct determination with luciferase luminescence using a biometer." Clinical Biochemistry 11(5): 190-193. <http://hdl.handle.net/2027.42/22719> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TDD-4GXVY2S-1/2/c1c33cb31688a69f2bfd3cd5cfdaeba6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22719 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=729160&dopt=citation | en_US |
dc.description.abstract | A method is described for the determination of low plasma levels of adenosine-5'-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 [mu]mol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 [plus-or-minus sign] 0.025 (10) [mu]mol/liter and rabbit plasma concentration were 0.07 [plus-or-minus sign] 0.05 (5) [mu]mol/liter. Several other possible applications of the method are discussed. | en_US |
dc.format.extent | 379595 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Plasma ADP levels: Direct determination with luciferase luminescence using a biometer | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology and Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | William Beaumont Hospital, Department of Surgery, Royal Oak, Michigan 48072, USA | en_US |
dc.contributor.affiliationother | William Beaumont Hospital, Department of Surgery, Royal Oak, Michigan 48072, USA | en_US |
dc.identifier.pmid | 729160 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22719/1/0000274.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0009-9120(78)80027-7 | en_US |
dc.identifier.source | Clinical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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