Plasma ADP levels: Direct determination with luciferase luminescence using a biometer

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dc.contributor.author Jabs, Clarence M. en_US
dc.contributor.author Ferrell, William J. en_US
dc.contributor.author Robb, Herbert J. en_US
dc.date.accessioned 2006-04-07T17:05:05Z
dc.date.available 2006-04-07T17:05:05Z
dc.date.issued 1978 en_US
dc.identifier.citation Jabs, Clarence M., Ferrell, William J., Robb, Herbert J. (1978)."Plasma ADP levels: Direct determination with luciferase luminescence using a biometer." Clinical Biochemistry 11(5): 190-193. <http://hdl.handle.net/2027.42/22719> en_US
dc.identifier.uri http://www.sciencedirect.com/science/article/B6TDD-4GXVY2S-1/2/c1c33cb31688a69f2bfd3cd5cfdaeba6 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/22719
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=729160&dopt=citation en_US
dc.description.abstract A method is described for the determination of low plasma levels of adenosine-5'-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 [mu]mol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 [plus-or-minus sign] 0.025 (10) [mu]mol/liter and rabbit plasma concentration were 0.07 [plus-or-minus sign] 0.05 (5) [mu]mol/liter. Several other possible applications of the method are discussed. en_US
dc.format.extent 379595 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language.iso en_US
dc.publisher Elsevier en_US
dc.title Plasma ADP levels: Direct determination with luciferase luminescence using a biometer en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Pathology en_US
dc.subject.hlbsecondlevel Dentistry en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Pathology and Biological Chemistry, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA en_US
dc.contributor.affiliationother William Beaumont Hospital, Department of Surgery, Royal Oak, Michigan 48072, USA en_US
dc.contributor.affiliationother William Beaumont Hospital, Department of Surgery, Royal Oak, Michigan 48072, USA en_US
dc.identifier.pmid 729160 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/22719/1/0000274.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1016/S0009-9120(78)80027-7 en_US
dc.identifier.source Clinical Biochemistry en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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