Lymphocyte transformation by pollen extracts and purified protein derivative (PPD) in leukocyte cultures of normal human subjects : I. Description of findings and conditions required
dc.contributor.author | Mathews, Kenneth P. | en_US |
dc.contributor.author | Pan, Pauline M. | en_US |
dc.contributor.author | Weisberg, Stephen C. | en_US |
dc.date.accessioned | 2006-04-07T17:10:03Z | |
dc.date.available | 2006-04-07T17:10:03Z | |
dc.date.issued | 1977-07 | en_US |
dc.identifier.citation | Mathews, Kenneth P., Pan, Pauline M., Weisberg, Stephen C. (1977/07)."Lymphocyte transformation by pollen extracts and purified protein derivative (PPD) in leukocyte cultures of normal human subjects : I. Description of findings and conditions required." Cellular Immunology 32(1): 120-134. <http://hdl.handle.net/2027.42/22880> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCF-4DMP814-1V/2/f2f319551a37d7155b627205ace64bc4 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22880 | |
dc.description.abstract | It is shown in a large number of experiments that short ragweed extract, mixed-grass pollen extract, and purified protein derivative (PPD) can produce very substantial tritiated thymidine uptake and lymphocyte blast transformation of peripheral blood leukocytes from normal individuals. Cord blood lymphocytes were also stimulated by pollen extracts. Differing conditions of culture probably explain the discrepancy between these results and many previous studies in which normal cells were not stimulated by these agents. Of particular importance are the use of human plasma in the cultures, restricted washing of the cells, the culture medium, and prolonged (8-day) cell culture times. Heparin has considerable influence on the results. | en_US |
dc.format.extent | 1197926 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Lymphocyte transformation by pollen extracts and purified protein derivative (PPD) in leukocyte cultures of normal human subjects : I. Description of findings and conditions required | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Montgomery Allergy Research Laboratory, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A | en_US |
dc.contributor.affiliationum | Montgomery Allergy Research Laboratory, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A | en_US |
dc.contributor.affiliationum | Montgomery Allergy Research Laboratory, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22880/1/0000444.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0008-8749(77)90187-3 | en_US |
dc.identifier.source | Cellular Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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