Thyroid control over biomembranes : Rat liver mitochondrial inner membranes
dc.contributor.author | Chen, Yii-Der Ida | en_US |
dc.contributor.author | Hoch, Frederic L. | en_US |
dc.date.accessioned | 2006-04-07T17:11:08Z | |
dc.date.available | 2006-04-07T17:11:08Z | |
dc.date.issued | 1977-06 | en_US |
dc.identifier.citation | Chen, Y. -D. Ida, Hoch, Frederic L. (1977/06)."Thyroid control over biomembranes : Rat liver mitochondrial inner membranes." Archives of Biochemistry and Biophysics 181(2): 470-483. <http://hdl.handle.net/2027.42/22915> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DN46Y3-198/2/f89dbc89f7ce779a9d51978c44f1b062 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22915 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=143239&dopt=citation | en_US |
dc.description.abstract | The effects of hypothyroidism and one injection of -thyroxine on oxidative phosphorylation and the composition of proteins and phospholipids were examined in vesicles prepared from rat liver mitochondria by digitonin extractions. At 30 [deg]C, the rates of ADP phosphorylation in sites I and II were below normal, and Mg2+-ATPase activity was greater than normal in vesicles from hypothyroid rats. At temperatures below 20 [deg]C and above 30 [deg]C, the Mg2+-ATPase was not accelerated above normal rates, a feature of temperature dependence shared by ADP phosphorylation (Chen, Y.-D. I., and Hoch, F. L., 1976, Arch. Biochem. Biophys. 172, 741-744). Respiration at 30 [deg]C was undiminished in hypothyroid vesicles, as were the flavin and cytochrome contents, and thyroxine administration corrected the phosphorylation rate at 30 [deg]C in 3 days without changing either respiration or electron-carrier contents. The 30 [deg]C phosphorylation defect comprised a decreased V and Km for ADP and a decrease in the number of phosphorylating sites (measured with oligomycin) that accounted for most of the decreased phosphorylation rates, either dependent on or independent of the adenine nucleotide carrier. Vesicles from hypothyroid rats were not detectably depleted in major protein subunits, but were abnormal in phospholipid fatty acid contents. Thyroxine injection corrected the low unsaturation index of the fatty acids and the membrane contents of linoleic acid and its fatty acyl metabolites. Hypothyroidism appears to affect oxidative phosphorylation through the altered inner membrane lipid environment, which implies that previously reported direct, reversible effects of thyroxine may mimic repletion of the membranes with unsaturated fatty acyl groups. | en_US |
dc.format.extent | 1399329 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Thyroid control over biomembranes : Rat liver mitochondrial inner membranes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Biological Chemistry and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Departments of Biological Chemistry and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 143239 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22915/1/0000480.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(77)90253-3 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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