Sensitive radioimmunoassay for digoxin in plasma and urine
dc.contributor.author | Wagner, John G. | en_US |
dc.contributor.author | Hallmark, Margarette R. | en_US |
dc.contributor.author | Sakmar, Ermelinda | en_US |
dc.contributor.author | Ayres, James W. | en_US |
dc.date.accessioned | 2006-04-07T17:11:11Z | |
dc.date.available | 2006-04-07T17:11:11Z | |
dc.date.issued | 1977-06 | en_US |
dc.identifier.citation | Wagner, J. G., Hallmark, M. R., Sakmar, E., Ayres, J. W. (1977/06)."Sensitive radioimmunoassay for digoxin in plasma and urine." Steroids 29(6): 787-807. <http://hdl.handle.net/2027.42/22917> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TC9-47P8XPG-7/2/4d252a1f6fe6e0e6f7c23604d8994958 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22917 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=910251&dopt=citation | en_US |
dc.description.abstract | A reproducible and sensitive radioimmunoassay for digoxin in either serum, plasma or urine is described. Using 0.5 ml of serum or plasma, the assay sensitivity is 0.05 ng of digoxin/ml. The antiserum and tracer solutions employed are available in a kit sold in the United States. All other reagents were prepared in the laboratory. The assay allows measurement of digoxin in plasma or serum for 96 hours after single 0.5 mg doses of digoxin; this is necessary in human bioavailability studies to accurately estimate the total area under the digoxin concentration, time curve from zero to infinite time. In contrast, with the kit assay, employing 0.2 ml of plasma or serum, it has been reported that the 12 hr serum digoxin levels, after single 0.5 mg doses, are, in most subjects, below the sensitivity limit (about 0.5 ng/ml) of the assay. | en_US |
dc.format.extent | 1388363 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Sensitive radioimmunoassay for digoxin in plasma and urine | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | College of Pharmacy and Upjohn Center for Clinical Pharmacology The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | College of Pharmacy and Upjohn Center for Clinical Pharmacology The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | College of Pharmacy and Upjohn Center for Clinical Pharmacology The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | School of Pharmacy, Oregon State University, Corvallis, Oregon 97331; College of Pharmacy and Upjohn Center for Clinical Pharmacology The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 910251 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22917/1/0000482.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0039-128X(77)90122-2 | en_US |
dc.identifier.source | Steroids | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.