Quantitative effects of unsaturated fatty acids in microbial mutants : VII. Influence of the acetylenic bond location on the effectiveness of acyl chains
dc.contributor.author | Lands, William E. M. | en_US |
dc.contributor.author | Ohlrogge, John B. | en_US |
dc.contributor.author | Robinson, John R. | en_US |
dc.contributor.author | Sacks, Roger W. | en_US |
dc.contributor.author | Barve, John A. | en_US |
dc.contributor.author | Gunstone, Frank D. | en_US |
dc.date.accessioned | 2006-04-07T17:12:19Z | |
dc.date.available | 2006-04-07T17:12:19Z | |
dc.date.issued | 1977-03-25 | en_US |
dc.identifier.citation | Lands, William E. M., Ohlrogge, John B., Robinson, John R., Sacks, Roger W., Barve, John A., Gunstone, Frank D. (1977/03/25)."Quantitative effects of unsaturated fatty acids in microbial mutants : VII. Influence of the acetylenic bond location on the effectiveness of acyl chains." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 486(3): 451-461. <http://hdl.handle.net/2027.42/22954> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1X-47F715C-9D/2/9eb43a2909e2cc05ae2511f4d48bee72 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22954 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=322722&dopt=citation | en_US |
dc.description.abstract | The ability of a series of 18 carbon acetylenic fatty acids to fulfill the unsaturated fatty acid requirements of Escherichia coli and Saccharomyces cerevisiae was investigated. Despite their high melting points (>40[deg]C), several isomers of the acetylenic fatty acids were as efficient or more efficient in supporting growth than the analogous fatty acid having a cis-double bond.The efficiencies of the different positional isomers in supporting cell proliferation varied from essentially 0 cells per fmol for the 2-5 and 13-17 isomers to high values when the acetylenic bond was near the center of the chain: e.g. 45 E. coli and 5.5 S. cerevisiae cells/fmol for the 10 isomer. A striking ineffectiveness of the 9 isomer was observed with E. coli. The 7, 8 and 10 isomers were at least 10-fold more efficient than any of the other positional isomers in supporting the growth of E. coli. In contrast, the 9 isomer was among the most effective acetylenic fatty acids tested with the yeast mutant.Chromatographic analysis of the extracted lipids indicated that each of the acetylenic isomers tested (except [Delta]2 and [Delta]3) could be esterified by the prokaryotic and eukaryotic microorganisms. The content of unsaturated plus cyclopropane acids observed when growth ceased in E. coli cultures supplemented with growth-limiting concentrations of the acetylenic fatty acids ranged from approx. 15 mol% for the 8 isomer to approx. 35 mol% for the 14 and 17 isomers. The 8-11 isomers were observed to be esterified predominantly at the two position in phosphatidylethanolamine of E. coli and in phosphatidylcholine of S. cerevisiae. | en_US |
dc.format.extent | 957389 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Quantitative effects of unsaturated fatty acids in microbial mutants : VII. Influence of the acetylenic bond location on the effectiveness of acyl chains | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | The Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | The Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | The Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Chemistry, St. Andrews University, St. Andrews, U.K. | en_US |
dc.contributor.affiliationother | Department of Chemistry, St. Andrews University, St. Andrews, U.K. | en_US |
dc.identifier.pmid | 322722 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22954/1/0000521.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2760(77)90095-9 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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