Adenine nucleotide translocation in liver mitochondria of hypothyroid rats
dc.contributor.author | Hoch, Frederic L. | en_US |
dc.date.accessioned | 2006-04-07T17:13:33Z | |
dc.date.available | 2006-04-07T17:13:33Z | |
dc.date.issued | 1977-01-30 | en_US |
dc.identifier.citation | Hoch, Frederic L. (1977/01/30)."Adenine nucleotide translocation in liver mitochondria of hypothyroid rats." Archives of Biochemistry and Biophysics 178(2): 535-545. <http://hdl.handle.net/2027.42/22994> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DYM9J4-1MJ/2/661048c402d271d2655f645d2e4abdba | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22994 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=138390&dopt=citation | en_US |
dc.description.abstract | In measurements using a disc filtration method, liver mitochondria obtained from hypothyroid rats translocate external ADP at 0 [deg]C via the atractyloside-sensitive carrier much more slowly than do mitochondria from normal rats, confirming the findings of Portnay et al. (Biochem. Biophys. Res. Commun. 55, 17, 1973). The hypothyroid mitochondria contain 60% more ATP + ADP than do mitochondria from normals, but the excess nucleotides are not exchangeable and so do not contribute to translocation. A decrease in the first-order rate constant accounts for the decreased velocity. Neither a decrease in the number of translocator sites nor changes in ADP phosphorylation or ATPase activity seem to account for the abnormal kinetics of translocation. Although the filtration method limits the maximal translocation rate observed in normal mitochondria at temperatures above 17 [deg]C that induce a fluid membrane state, no such transition is seen in mitochondria from hypothyroid rats up to 35 [deg]C, indicating that the translocator is in an altered environment in hypothyroidism. Injecting a hypothyroid rat once with -thyroxine corrects the abnormal compartmentation and produces a temperature-rate relationship like that in normal mitochondria in 3 days, a period which would accommodate the hormone actions reported on translation, membrane phospholipid synthesis, or fatty acid desaturation. | en_US |
dc.format.extent | 1047902 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Adenine nucleotide translocation in liver mitochondria of hypothyroid rats | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine and Biological Chemistry, The University of Michigan, 7696 Kresge Building, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.identifier.pmid | 138390 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22994/1/0000562.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(77)90224-7 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.