Role of proton dissociation in the transport of acidic amino acids by the Ehrlich ascites tumor cells
dc.contributor.author | Garcia-Sancho, Javier | en_US |
dc.contributor.author | Sanchez, Ana | en_US |
dc.contributor.author | Christensen, Halvor N. | en_US |
dc.date.accessioned | 2006-04-07T17:13:41Z | |
dc.date.available | 2006-04-07T17:13:41Z | |
dc.date.issued | 1977-01-21 | en_US |
dc.identifier.citation | Garcia-Sancho, Javier, Sanchez, Ana, Christensen, Halvor N. (1977/01/21)."Role of proton dissociation in the transport of acidic amino acids by the Ehrlich ascites tumor cells." Biochimica et Biophysica Acta (BBA) - Biomembranes 464(2): 295-312. <http://hdl.handle.net/2027.42/22998> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1T-47T1MGM-P4/2/c510e433eebb82ff0b11424b66ef3662 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/22998 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12815&dopt=citation | en_US |
dc.description.abstract | The pH profile for the uptake of -glutamic acid by the Ehrlich ascites tumor cell arises largely as a sum of the decline with falling pH of a slow, Na+-dependent uptake by System A, and an increasing uptake by Na+-independent System L. The latter maximizes at about pH 4.5, following approximately the titration curve of the distal carboxyl group. This shift in route of uptake was verified by (a) a declining Na+-dependent component. (b) an almost corresponding decline in the 2-(methylamino)-isobutyric acid-inhibitable component, (c) a rising component inhibited by 2-aminonorbornane-2-carboxylic acid. Other amino acids recognized as principally reactive with Systems A or L yielded corresponding inhibitory effects with some conspicuous exceptions: 2-Aminoisobutyric acid and even glycine become better substrates of System L as the pH is lowered; hence their inhibitory action on glutamic acid uptake is not lost. The above results were characterized by generally consistent relations among the half-saturation concentrations of the interacting amino acids with respect to: their own uptake, their inhibition of the uptake, one by another, and their trans stimulation of exodus, one by another.A small Na+-dependent component of uptake retained by -glutamic acid but not by-glutamic acid at pH 4.5 is inhibitable by methionine but by neither 2-(methylamino)-isobutyric acid nor the norbornane amino acid. We provisionally identified this component with System ASC, which transports -glutamine throughout the pH range studied. No transport activity specific to the anionic amino acids was detected, and the unequivocally anionic cysteic acid showed neither significant mediated uptake nor inhibition of the uptake of glutamic acid or of the norbornane amino acid. | en_US |
dc.format.extent | 1146125 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Role of proton dissociation in the transport of acidic amino acids by the Ehrlich ascites tumor cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.identifier.pmid | 12815 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/22998/1/0000566.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2736(77)90005-0 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.