Induction of tyrosine aminotransferase and amino acid transport in rat hepatoma cells by insulin and the insulin-like growth factor, multiplication-stimulating activity : Mediation by insulin and multiplication-stimulating activity receptors
dc.contributor.author | Heaton, Joanne H. | en_US |
dc.contributor.author | Schilling, Ellen E. | en_US |
dc.contributor.author | Gelehrter, Thomas D. | en_US |
dc.contributor.author | Rechler, Matthew M. | en_US |
dc.contributor.author | Spencer, Carolyn J. | en_US |
dc.contributor.author | Peter Nissley, S. | en_US |
dc.date.accessioned | 2006-04-07T17:21:13Z | |
dc.date.available | 2006-04-07T17:21:13Z | |
dc.date.issued | 1980-10-01 | en_US |
dc.identifier.citation | Heaton, Joanne H., Schilling, Ellen E., Gelehrter, Thomas D., Rechler, Matthew M., Spencer, Carolyn J., Peter Nissley, S. (1980/10/01)."Induction of tyrosine aminotransferase and amino acid transport in rat hepatoma cells by insulin and the insulin-like growth factor, multiplication-stimulating activity : Mediation by insulin and multiplication-stimulating activity receptors." Biochimica et Biophysica Acta (BBA) - General Subjects 632(2): 192-203. <http://hdl.handle.net/2027.42/23133> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1W-47MNXT1-1GN/2/dceb7eac8d689362ea83ca893f41fce4 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23133 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6106509&dopt=citation | en_US |
dc.description.abstract | Insulin stimulates a 2-fold increase in the amount of tyrosine aminotransferase and a 5-10-fold increase in the rate of amino acid transport in dexamethasone-treated rat hepatoma cells. In order to determine whether these effects are mediated by insulin receptors or receptors for insulin-like growth factors, we have examined the binding of 125I-labeled insulin and 125I-labeled multiplication-stimulating activity, a prototype insulin-like growth factor, and compared the biological effects of these polypeptides. Insulin and multiplication-stimulating activity cause an identical increase in transaminase activity and transport velocity; half-maximal biological effects were observed at 35 ng/ml (5.5 nM) insulin and 140 ng/ml multiplication-stimulating activity. The hepatoma cells display typical insulin receptors of appropriate specificity; half-maximal displacement of tracer insulin binding occured at 33 ng/ml unlabeled insulin, but only at 2500 ng/ml unlabeled multiplication-stimulating activity. Specific multiplication-stimulating activity receptors also were demonstrated with which insulin did not interact even at 10 [mu]g/ml. Half-maximal displacement of tracer multiplication-stimulating activity occured at 200 ng/ml unlabeled multiplication-stimulating activity. We conclude that insulin cannot act via the multiplication-stimulating activity receptor and presumably acts via typical insulin receptors. The effects of multiplication-stimulating activity on enzyme induction and amino acid transport are probably mediated primarily via the multiplication-stimulating activity receptor. | en_US |
dc.format.extent | 836111 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Induction of tyrosine aminotransferase and amino acid transport in rat hepatoma cells by insulin and the insulin-like growth factor, multiplication-stimulating activity : Mediation by insulin and multiplication-stimulating activity receptors | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Department of Michigan, Ann Arbor, MI 48109, U.S.A.; Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Human Genetics, Department of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Biochemical Pharmacology, NIAMDD, Bethesda, MD 20205, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Biochemical Pharmacology, NIAMDD, Bethesda, MD 20205, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Human Genetics, Department of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Metabolism Branch, National Cancer Institute, Bethesda, MD 20205, U.S.A. | en_US |
dc.identifier.pmid | 6106509 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23133/1/0000057.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0304-4165(80)90077-X | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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