A revised fluorometric assay for Gaucher's disease using conduritol-[beta]-epoxide with liver as the source of [beta]-glucosidase
dc.contributor.author | Daniels, Lydia B. | en_US |
dc.contributor.author | Glew, Robert H. | en_US |
dc.contributor.author | Radin, Norman S. | en_US |
dc.contributor.author | Vunnam, Ranga R. | en_US |
dc.date.accessioned | 2006-04-07T17:21:32Z | |
dc.date.available | 2006-04-07T17:21:32Z | |
dc.date.issued | 1980-09-25 | en_US |
dc.identifier.citation | Daniels, Lydia B., Glew, Robert H., Radin, Norman S., Vunnam, Ranga R. (1980/09/25)."A revised fluorometric assay for Gaucher's disease using conduritol-[beta]-epoxide with liver as the source of [beta]-glucosidase." Clinica Chimica Acta 106(2): 155-163. <http://hdl.handle.net/2027.42/23143> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T57-47RB2KC-264/2/c8633610ce2c7b573f4dbddf170e0b18 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23143 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6773704&dopt=citation | en_US |
dc.description.abstract | To date, enzymatic diagnosis of Gaucher's disease via a fluorometric assay procedure which utilizes 4-methylumbelliferyl-[beta]--glucopyranoside as a substrate has not been possible when liver serves as the source of enzyme since currently employed fluorometric procedures cannot adequately differentiate between a broad-specificity [beta]-glucosidase and lysosomal glucocerebrosidase activities in crude extracts of liver. Incorporation of conduritol-[beta]-epoxide into the incubation medium for the fluorometric assay allows one to selectively measure the glucocerebrosidase activity present in a given liver extract. In five cases of Gaucher's disease this revised fluorometric procedure proved as effective as the assay procedure which utilizes authentic, radiolabeled glucocerebroside as the substrate in demonstrating a deficiency of glucocerebrosidase activity in liver. | en_US |
dc.format.extent | 790204 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A revised fluorometric assay for Gaucher's disease using conduritol-[beta]-epoxide with liver as the source of [beta]-glucosidase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Psychiatry, Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Psychiatry, Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, U.S.A. | en_US |
dc.identifier.pmid | 6773704 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23143/1/0000067.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0009-8981(80)90168-0 | en_US |
dc.identifier.source | Clinica Chimica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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