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A method for the chemical synthesis of 14C-labeled fatty acyl coenzyme A's of high specific activity

dc.contributor.authorBishop, James E.en_US
dc.contributor.authorHajra, Amiya K.en_US
dc.date.accessioned2006-04-07T17:22:47Z
dc.date.available2006-04-07T17:22:47Z
dc.date.issued1980-08en_US
dc.identifier.citationBishop, James E., Hajra, Amiya K. (1980/08)."A method for the chemical synthesis of 14C-labeled fatty acyl coenzyme A's of high specific activity." Analytical Biochemistry 106(2): 344-350. <http://hdl.handle.net/2027.42/23182>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DX47FN-6V/2/0d3c31c7fbb666d862d1e732f2a11054en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/23182
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7447002&dopt=citationen_US
dc.description.abstractA simple and reliable method, based on that described by W. Seubert (1960, Biochem Prep. 7, 80-83), has been developed for the chemical synthesis of radioactive acyl coenzyme A's. 1-14C-labeled fatty acids (palmitic, oleic, and linoleic) are converted to their acyl chlorides with oxalyl chloride. The [1-14C]acyl chlorides are then condensed with a two- to three-fold molar excess of coenzyme A in a bicarbonate-buffered tetrahydrofuran solution to form the corresponding [1-14C]acyl coenzyme A's. The overall yields are near 75%, and the purities are greater than 90% based on spectral, chromatographic, and enzymatic properties.en_US
dc.format.extent549406 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA method for the chemical synthesis of 14C-labeled fatty acyl coenzyme A's of high specific activityen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumMental Health Research Institute University of Michigan, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumMental Health Research Institute University of Michigan, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid7447002en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/23182/1/0000109.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(80)90531-Xen_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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