Histological localization of binding sites of [alpha]-bungarotoxin and of antibodies specific to acetylcholine receptor in goldfish optic nerve and tectum
dc.contributor.author | Schwartz, Michal | en_US |
dc.contributor.author | Axelrod, Daniel | en_US |
dc.contributor.author | Feldman, Eva L. | en_US |
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.date.accessioned | 2006-04-07T17:23:11Z | |
dc.date.available | 2006-04-07T17:23:11Z | |
dc.date.issued | 1980-07-21 | en_US |
dc.identifier.citation | Schwartz, M., Axelrod, D., Feldman, E. L., Agranoff, B. W. (1980/07/21)."Histological localization of binding sites of [alpha]-bungarotoxin and of antibodies specific to acetylcholine receptor in goldfish optic nerve and tectum." Brain Research 194(1): 171-180. <http://hdl.handle.net/2027.42/23195> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYR-485PB1G-139/2/a0ab8664aab0381a18088175bfd41b59 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23195 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7378835&dopt=citation | en_US |
dc.description.abstract | Goldfish optic nerve as well as ganglion cell neurites grown in culture selectively bind rhodamine-labeled [alpha]-bungarotoxin following tissue fixation. Binding is competed for by unlabeled bungarotoxin, by carbamylcholine and tubocurarine, but not by atropine. In cross-sections, the label is seen confined to axonal bundles. The binding is not detectable without prior fixation and is very faint in brain sections, even after fixation.To further establish the nature of the binding, immunocytochemical studies were performed, taking advantage of a high cross-reactivity found between goldfish brain and antibodies against eel acetylcholine receptor (AChR). Antigenic sites were detected by an indirect unlabeled antibody complexed to horseradish peroxidase. Anti-AChR antibody binding to optic nerve and neurites in culture correlated with that seen with [alpha]-bungarotoxin. Binding of anti-AChR was observed in the brain, and was reduced in the denervated tectum following unilateral optic nerve crush or enucleation.The results are discussed in relation to functions of receptor proteins in the retinotectal system. | en_US |
dc.format.extent | 1344397 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Histological localization of binding sites of [alpha]-bungarotoxin and of antibodies specific to acetylcholine receptor in goldfish optic nerve and tectum | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory and (D.A.) Biophysics Research Division, Department of Physics, University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory and (D.A.) Biophysics Research Division, Department of Physics, University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory and (D.A.) Biophysics Research Division, Department of Physics, University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory and (D.A.) Biophysics Research Division, Department of Physics, University of Michigan, Ann Arbor, Mich. 48109, U.S.A. | en_US |
dc.identifier.pmid | 7378835 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23195/1/0000122.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-8993(80)91326-8 | en_US |
dc.identifier.source | Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.