Purification of bovine liver microsomal NADH-cytochrome reductase using affinity chromatography
dc.contributor.author | Schafer, Dorothy A. | en_US |
dc.contributor.author | Hultquist, Donald E. | en_US |
dc.date.accessioned | 2006-04-07T17:23:20Z | |
dc.date.available | 2006-04-07T17:23:20Z | |
dc.date.issued | 1980-07-16 | en_US |
dc.identifier.citation | Schafer, Dorothy A., Hultquist, Donald E. (1980/07/16)."Purification of bovine liver microsomal NADH-cytochrome reductase using affinity chromatography." Biochemical and Biophysical Research Communications 95(1): 381-387. <http://hdl.handle.net/2027.42/23200> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DN95M1-10S/2/a9e05830baa2ffa5e7ab35ce4b1a806c | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23200 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7417264&dopt=citation | en_US |
dc.description.abstract | Microsomal NADH-cytochrome reductase has been purified from bovine liver by an improved procedure which employs affinity chromatography on ADP-agarose in combination with anion exchange chromatography. The reductase was extracted from a 105,000 x microsomal pellet with Triton X-100. The overall purification from isolated microsomes was 98-fold and the yield was 10%. The preparation was nearly homogeneous on SDS-PAGE. This procedure requires less time and effort than previously described procedures. Partially purified cytochrome is also obtained. | en_US |
dc.format.extent | 586621 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Purification of bovine liver microsomal NADH-cytochrome reductase using affinity chromatography | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 7417264 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23200/1/0000127.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(80)90749-4 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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