Transport of hexoses, potassium and neutral amino acids into capillaries isolated from bovine retina

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dc.contributor.author Betz, A. Lorris en_US
dc.contributor.author Goldstein, Gary W. en_US
dc.date.accessioned 2006-04-07T17:25:11Z
dc.date.available 2006-04-07T17:25:11Z
dc.date.issued 1980-05 en_US
dc.identifier.citation Betz, A. Lorris, Goldstein, Gary W. (1980/05)."Transport of hexoses, potassium and neutral amino acids into capillaries isolated from bovine retina." Experimental Eye Research 30(5): 593-605. <http://hdl.handle.net/2027.42/23260> en_US
dc.identifier.uri http://www.sciencedirect.com/science/article/B6WFD-4BMD2GF-T/2/395ec87d58d0451ce61caafdc6749012 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/23260
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7409014&dopt=citation en_US
dc.description.abstract Capillaries were isolated from bovine retina by homogenization and glass bead filtration in order to study their ability to transport certain solutes. Viability of the microvessels was demonstrated by their ability to maintain linear rates of substrate oxidation for more than two hours. Hexose uptake (measured using 3-o-methyl--glucose) could be inhibited by cytochalasin B, phloretin and phlorizin, but not by 2,4-dinitrophenol or ouabain. -Glucose, 2-deoxy--glucose, -mannose, -galactose and -xylose inhibited 3-o-methyl--glucose uptake, while -glucose, -ribose and -fructose did not. When incubated at 37[deg]C with 5 m-glucose, the microvessels contained much more free -glucose than -glucose metabolites. Thus, transport was not rate limiting for metabolism. -Glucose entered capillaries more slowly than other hexoses and served as a marker for simple diffusion of sugars into the cells. 86Rb+ was used as an indicator of K+ transport activity. Uptake of 86Rb+ was temperature sensitive and markedly inhibited by I m ouabain, thus indicating the presence of an active K+ transport system. Na+-dependent amino acid transport was demonstrated using [alpha]-(methylamino)isobutyric acid as a model substrate. Capillary uptake of this neutral amino acid analogue was inhibited after abolishing the Na+ gradient with 1 m-ouabain. Uptake of the organic anion p-aminohippuric acid (PAH) was slightly greater than uptake of the extracellular marker sucrose. There was a small inhibition of PAH uptake by fluorescein and penicillin but not by probenicid. Our results indicate the presence of several transport processes in retinal capillary endothelial cells which may be important for the maintenance of homeostasis within the retina. en_US
dc.format.extent 2333929 bytes
dc.format.extent 3118 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language.iso en_US
dc.publisher Elsevier en_US
dc.title Transport of hexoses, potassium and neutral amino acids into capillaries isolated from bovine retina en_US
dc.rights.robots IndexNoFollow en_US
dc.subject.hlbsecondlevel Public Health en_US
dc.subject.hlbsecondlevel Ophthalmology en_US
dc.subject.hlbsecondlevel Neurosciences en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbtoplevel Science en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Neurology, University of Michigan, Ann Arbor, MI. 48109, U.S.A.: Department of Pediatrics, University of Michigan, Ann Arbor, MI. 48109, U.S.A. en_US
dc.contributor.affiliationum Department of Neurology, University of Michigan, Ann Arbor, MI. 48109, U.S.A.: Department of Pediatrics, University of Michigan, Ann Arbor, MI. 48109, U.S.A. en_US
dc.identifier.pmid 7409014 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/23260/1/0000193.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1016/0014-4835(80)90042-1 en_US
dc.identifier.source Experimental Eye Research en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
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