Concurrent isolation of granulocytes and lymphocytes with unaltered permeability, energy state, and metabolic capacity in vitro
dc.contributor.author | Cullen, Edward I. | en_US |
dc.contributor.author | Medzihradsky, Fedor | en_US |
dc.date.accessioned | 2006-04-07T17:25:40Z | |
dc.date.available | 2006-04-07T17:25:40Z | |
dc.date.issued | 1980-04 | en_US |
dc.identifier.citation | Cullen, Edward I., Medzihradsky, Fedor (1980/04)."Concurrent isolation of granulocytes and lymphocytes with unaltered permeability, energy state, and metabolic capacity in vitro." Biochemical Medicine 23(2): 133-143. <http://hdl.handle.net/2027.42/23272> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B7G7P-4C4NX2F-HS/2/12031653b56042b886e911174f435ffe | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23272 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7396884&dopt=citation | en_US |
dc.description.abstract | A method has been developed based on sedimentation, discontinuous density gradient centrifugation, and differential lysis for the concurrent isolation from the same sample of blood of granulocytes and lymphocytes with yields of 27 and 38%, respectively. The isolated cellular fractions were characterized by a lack of cross-contamination and by the virtual absence of erythrocytes and platelets. Plasma membrane integrity, energy status, and metabolic capacity of the isolated cells were assessed by the cell contents of potassium, sodium, ATP, and by the uptake of oxygen. The respective values for these parameters of cell viability compared favorably with previously reported data for separately isolated granulocytes and lymphocytes. The cellular characteristics determined immediately after the isolation of the cells were maintained throughout a 2-hr period at room temperature. | en_US |
dc.format.extent | 686118 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Concurrent isolation of granulocytes and lymphocytes with unaltered permeability, energy state, and metabolic capacity in vitro | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. | en_US |
dc.identifier.pmid | 7396884 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23272/1/0000209.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-2944(80)90064-2 | en_US |
dc.identifier.source | Biochemical Medicine | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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