A comparison of cholesteryl oleate and 19-iodocholesteryl oleate as substrates for adrenal cholesterol esterase
dc.contributor.author | Nordblom, Gerald D. | en_US |
dc.contributor.author | Schappa, L. W. | en_US |
dc.contributor.author | Floyd, E. E. | en_US |
dc.contributor.author | Langdon, R. B. | en_US |
dc.contributor.author | Counsell, Raymond E. | en_US |
dc.date.accessioned | 2006-04-07T17:25:59Z | |
dc.date.available | 2006-04-07T17:25:59Z | |
dc.date.issued | 1980-04 | en_US |
dc.identifier.citation | Nordblom, G. D., Schappa, L. W., Floyd, E. E., Langdon, R. B., Counsell, R. E. (1980/04)."A comparison of cholesteryl oleate and 19-iodocholesteryl oleate as substrates for adrenal cholesterol esterase." Journal of Steroid Biochemistry 13(4): 463-466. <http://hdl.handle.net/2027.42/23282> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B73GT-48CX3HV-19/2/17dbdda74ad144b899c2ea374e2796c2 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23282 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7392624&dopt=citation | en_US |
dc.description.abstract | Radiolabeled 19-iodocholesterol is widely used to obtain images of human adrenals. We have shown in rats and mice that 85-95% of the radiolabel is present in the esterified form. Using rat adrenal cytosolic cholesterol esterase, the kinetic parameters KM and V were determined for commercially available cholesteryl [1-14C]-oleate (I) and [125I]-19-iodocholesteryl oleate (II). The KM and V were found to be 16.2 [mu]M and 602 pmol/min/mg prot. respectively for I compared to 76.2 [mu]M and 37.6 pmol/min/mg prot. for II. Since V/KM is 37.3 ml/min/mg prot. for the normal ester and 0.49 ml/min/mg prot. for the iodinated analog, it appears that the normal substrate is 76 times more specific than II. In addition, direct competition experiments were run in which 67 [mu]M I was used as substrate. The addition of 65 and 325 [mu]M 19-iodocholesteryl oleate caused 20 and 49% inhibition, respectively. On the basis of these studies, it is not surprising that 19-iodocholesterol would accumulate in the adrenals in an esterified form and thus provide an effective agent for adrenal visualization. | en_US |
dc.format.extent | 417261 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A comparison of cholesteryl oleate and 19-iodocholesteryl oleate as substrates for adrenal cholesterol esterase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.identifier.pmid | 7392624 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23282/1/0000219.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-4731(80)90356-8 | en_US |
dc.identifier.source | Journal of Steroid Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.