Paired-ion high-performance liquid chromatographic assay for plasma choline
dc.contributor.author | Buchanan, Douglas N. | en_US |
dc.contributor.author | Fucek, Franz R. | en_US |
dc.contributor.author | Domino, Edward F. | en_US |
dc.date.accessioned | 2006-04-07T17:26:13Z | |
dc.date.available | 2006-04-07T17:26:13Z | |
dc.date.issued | 1980-03-14 | en_US |
dc.identifier.citation | Douglas N., Buchanan, Franz R Fucek, , Edward F Domino, (1980/03/14)."Paired-ion high-performance liquid chromatographic assay for plasma choline." Journal of Chromatography B: Biomedical Sciences and Applications 181(3-4): 329-335. <http://hdl.handle.net/2027.42/23290> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TG9-44BNNBY-2X/2/3362ce1a9581e145d6a05a1de6d1c4e0 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23290 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7391149&dopt=citation | en_US |
dc.description.abstract | Choline was isolated from deproteinized plasma by cation-exchange chromatography. Isolated choline was directly converted to the 3,5-dinitrobenzoate derivative and was analyzed by paired-ion high-performance liquid chromatography with UV detection at 254 nm. An internal standard, 3-hydroxy-N,N,N-trimethylpropanaminium iodide was used for quantitation of plasma choline.Linearity was achieved from 1-500 nmole/ml with a reproducibility of +/- 6%. Plasma choline concentrations below 1 nmole/ml could not be accurately measured while plasma choline concentrations in the [mu]mole/ml range deviated from linearity. | en_US |
dc.format.extent | 618218 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Paired-ion high-performance liquid chromatographic assay for plasma choline | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The University of Michigan, Ann Arbor, Mich. 48109 U.S.A. | en_US |
dc.contributor.affiliationum | The University of Michigan, Ann Arbor, Mich. 48109 U.S.A. | en_US |
dc.contributor.affiliationother | Department of Pharmacology, Lafayette Clinic, 951 E. Lafayette, Detroit, Mich. 48207 U.S.A. | en_US |
dc.identifier.pmid | 7391149 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23290/1/0000227.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0378-4347(00)81135-4 | en_US |
dc.identifier.source | Journal of Chromatography B: Biomedical Sciences and Applications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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