Viral DNA synthesis is required for the efficient expression of specific herpes simplex virus type 1 mRNA species
dc.contributor.author | Holland, Louis E. | en_US |
dc.contributor.author | Anderson, Kevin P. | en_US |
dc.contributor.author | Shipman, Charles Jr. | en_US |
dc.contributor.author | Wagner, Edward K. | en_US |
dc.date.accessioned | 2006-04-07T17:27:08Z | |
dc.date.available | 2006-04-07T17:27:08Z | |
dc.date.issued | 1980-02 | en_US |
dc.identifier.citation | Holland, Louis E., Anderson, Kevin P., Shipman, Jr., Charles, Wagner, Edward K. (1980/02)."Viral DNA synthesis is required for the efficient expression of specific herpes simplex virus type 1 mRNA species." Virology 101(1): 10-24. <http://hdl.handle.net/2027.42/23320> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WXR-4BSD318-7M/2/fd984a23351858328eb7355b18dc1594 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23320 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6243816&dopt=citation | en_US |
dc.description.abstract | Inhibition of HSV-1 DNA synthesis with either arabinosyladenine plus the adenosine deaminase inhibitor pentostatin, or with arabinosylthymine, showed a viral mRNA population identical to that seen prior to viral DNA replication (early) by the criteria of quantitative hybridization, specific mRNA species identifiable by Southern blot hybridization of size-fractionated RNA, and migration of polypeptides resolved by in vitro translation of purified viral mRNA. The amount of viral mRNA associated with infected cell polyribosomes was determined by quantitative DNA excess solution hybridization. At 2 hr postinfection (p.i.) (before viral DNA synthesis) and in drug-treated cells at 6 hr p.i., the majority of the polyadenylated RNA was cell specific with some virus-specific RNA detectable. In contrast, at 6 hr p.i., in the absence of drugs (during maximum viral DNA synthesis), nearly all the polyadenylated polyribosomal RNA was viral. Blot hybridization of size-fractionated viral RNA confirmed several specific differences between the viral mRNA species occurring before and after HSV-1 DNA synthesis, which have been reported previously from this laboratory. These differences also were reflected in the in vitro translation products encoded by the viral mRNAs. The mRNA species and the encoded polypeptides that were present in the absence of viral DNA synthesis are a subset of those viral mRNA species and polypeptides expressed in the presence of viral DNA synthesis. The viral mRNA species fall into several groups based on their relative abundance at various times of infection. These data suggest that, in the normal virus infection cycle, the onset of viral DNA synthesis is necessary for normal expression of later viral genes. | en_US |
dc.format.extent | 3093383 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Viral DNA synthesis is required for the efficient expression of specific herpes simplex virus type 1 mRNA species | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Oral Biology and the Dental Research Institute, School of Dentistry, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Department of MolecularBiology and Biochemistry, University of California, Irvine, Irvine, California 92717, USA | en_US |
dc.contributor.affiliationother | Department of MolecularBiology and Biochemistry, University of California, Irvine, Irvine, California 92717, USA | en_US |
dc.contributor.affiliationother | Department of MolecularBiology and Biochemistry, University of California, Irvine, Irvine, California 92717, USA | en_US |
dc.identifier.pmid | 6243816 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23320/1/0000259.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0042-6822(80)90479-1 | en_US |
dc.identifier.source | Virology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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