Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography
dc.contributor.author | Blake, Diane A. | en_US |
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.date.accessioned | 2006-04-07T17:27:14Z | |
dc.date.available | 2006-04-07T17:27:14Z | |
dc.date.issued | 1980-02 | en_US |
dc.identifier.citation | Blake, Diane A., Goldstein, Irwin J. (1980/02)."Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography." Analytical Biochemistry 102(1): 103-109. <http://hdl.handle.net/2027.42/23323> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DYTNJ8-111/2/12d68b2809b3eaae642a1b55e082b39e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23323 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7356144&dopt=citation | en_US |
dc.description.abstract | Bandeiraea simplicifolia I (BS I) isolectins, immobilized on Sepharose beads, specifically retarded low molecular weight ligands containing terminal [alpha]--galactopyranosyl and 2-acetamido-2-deoxy-[alpha]--galactopyranosyl residues. A BS I lectin-Sepharose column has been used to perform very efficient separations of structurally homologous sugar nucleotides and oligosaccharides. For example, UDP-glucose and UDP-galactose (and the corresponding 2-acetamido-2-deoxy derivatives) were separated by as much as three column volumes by a BS I lectin-Sepharose column. This column has also been used to resolve the alditols of lactose ([beta]--Galp-(1 --> 4)--Glc) and melibiose ([alpha]--Galp-(1 --> 6)--Glc), to analyze the radiochemical purity of UDP-galactose and UDP-N-acetyl--galactosamine preparations, and to analyze the relative proportions of UDP-N-acetyl--glucosamine and UDP-N-acetyl--galactosamine in the UDP-N-acetyl--hexosamine pool isolated from cultured cells. | en_US |
dc.format.extent | 578009 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 7356144 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23323/1/0000262.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(80)90324-3 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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