Interaction of monocytes with tumor cells coated with complement with or without antibody
dc.contributor.author | Kessler, Howard B. | en_US |
dc.contributor.author | LoBuglio, Albert F. | en_US |
dc.date.accessioned | 2006-04-07T17:27:24Z | |
dc.date.available | 2006-04-07T17:27:24Z | |
dc.date.issued | 1980-02 | en_US |
dc.identifier.citation | Kessler, Howard B., LoBuglio, Albert F. (1980/02)."Interaction of monocytes with tumor cells coated with complement with or without antibody." Cellular Immunology 49(2): 352-359. <http://hdl.handle.net/2027.42/23329> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCF-4DKMD7V-H2/2/ec40e6fcf7da0ccf0adab09c5a0e25e6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23329 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7353213&dopt=citation | en_US |
dc.description.abstract | Raji, a human B lymphoblastoid cell line has the ability to activate the complement cascade by alternate pathway mechanisms with subsequent fixation of C3 to receptors on the Raji cell membrane. Using this property, we examined the role that complement plays in mediating a cytolytic event between human peripheral blood monocytes and Raji cells coated with C3b, antibody, or both. Presence of C3 was confirmed by immune adherence. IgG bound to the Raji membrane was quantitated using I125 Staphylococcal protein A assay. The presence of alternate pathway-activated C3 on Raji cells failed to produce monocyte-mediated cytotoxicity. These same target cells subsequently coated with antibody concentration ranging from 200 to >600,000 SPA molecules per Raji cell produced neither enhancement nor inhibition of antibody-dependent, cell-mediated cytotoxicity (ADCC). ADCC was enhanced by complement when complement activation and binding of C3 to the cell surface occurred by classical pathway mechanisms. ADCC of 32% +/- 3.2 occurred with undiluted antiserum (625,000 SPA molecules bound/Raji cell) with enhancement to 52% +/- 1.1 in the presence of C3. IgG inhibition of ADCC was unaffected by the presence of membrane-bound C3. | en_US |
dc.format.extent | 568164 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Interaction of monocytes with tumor cells coated with complement with or without antibody | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Simpson Memorial Research Institute, University of Michigan, Ann Arbor, Michigan 48109, USA; Division of Hematology and Oncology, Ohio State University, College of Medicine, Columbus, Ohio 43210, USA | en_US |
dc.contributor.affiliationother | Division of Hematology and Oncology, Ohio State University, College of Medicine, Columbus, Ohio 43210, USA; Division of Hematology and Oncology, Ohio State University, College of Medicine, Columbus, Ohio 43210, USA | en_US |
dc.identifier.pmid | 7353213 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23329/1/0000269.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0008-8749(80)90036-2 | en_US |
dc.identifier.source | Cellular Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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