Immunological studies of the sperm-specific phosphoglycerate kinase-2 of mice
dc.contributor.author | Erickson, Robert P. | en_US |
dc.contributor.author | Harper, Kenneth J. | en_US |
dc.contributor.author | Menge, Alan C. | en_US |
dc.contributor.author | Lee, Chi-Yu Gregory | en_US |
dc.date.accessioned | 2006-04-07T17:32:18Z | |
dc.date.available | 2006-04-07T17:32:18Z | |
dc.date.issued | 1979-10 | en_US |
dc.identifier.citation | Erickson, Robert P., Harper, Kenneth, Menge, Alan, Lee, Chi-yu (1979/10)."Immunological studies of the sperm-specific phosphoglycerate kinase-2 of mice." Journal of Reproductive Immunology 1(3): 185-191. <http://hdl.handle.net/2027.42/23483> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T8W-476M29M-33/2/48423fde1cc1e83a17e04f605d81b42a | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23483 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=551176&dopt=citation | en_US |
dc.description.abstract | Antiserum was prepared to 500-fold purified PGK-2A from mouse testes. Despite the apparent high purity of the immunizing antigen, double diffusion analyses showed two precipitin lines, one of which can be shown to correspond to PGK-2. An immunoabsorbent column of liver homogenate coupled to cyanogen bromide-activated Sepharose was used to remove the contaminating antibody. Both immuno-inactivation and double diffusion analyses demonstrated that the PGK-2 antigen appeared in testes between days 30 and 34 of postnatal development. The antisera prepared against highly purified PGK-2A did not disclose any immunological differences between PGK-2A, PGK-2B, and PGK-2C either by double diffusion analyses or quantitative immunoprecipitation. Antisera to PGK-2 did not inhibit in vitro fertilization of zona-free hamster ova by mouse spermatozoa. | en_US |
dc.format.extent | 558322 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Immunological studies of the sperm-specific phosphoglycerate kinase-2 of mice | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Obstetrics and Gynecology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA;Department of Pediatrics, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Obstetrics and Gynecology, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationother | Laboratories of Environmental Mutagenesis and Biochemical Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, U.S.A. | en_US |
dc.identifier.pmid | 551176 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23483/1/0000436.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0165-0378(79)90019-6 | en_US |
dc.identifier.source | Journal of Reproductive Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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