Comparative studies of wild-type and cold-mutant (temperature-sensitive) influenza viruses: Nonrandom reassortment of genes during preparation of live virus vaccine candidates by recombination at 25[deg] between recent H3N2 and H1N1 epidemic strains and cold-adapted A/Ann Arbor/6/60
dc.contributor.author | Cox, Nancy J. | en_US |
dc.contributor.author | Maassab, Hunein F. | en_US |
dc.contributor.author | Kendal, Alan P. | en_US |
dc.date.accessioned | 2006-04-07T17:33:17Z | |
dc.date.available | 2006-04-07T17:33:17Z | |
dc.date.issued | 1979-08 | en_US |
dc.identifier.citation | Cox, Nancy J., Maassab, Hunein F., Kendal, Alan P. (1979/08)."Comparative studies of wild-type and cold-mutant (temperature-sensitive) influenza viruses: Nonrandom reassortment of genes during preparation of live virus vaccine candidates by recombination at 25[deg] between recent H3N2 and H1N1 epidemic strains and cold-adapted A/Ann Arbor/6/60." Virology 97(1): 190-194. <http://hdl.handle.net/2027.42/23515> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WXR-4CJ5V6D-161/2/8bedfd489575c185221bb006f4063d7d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23515 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=473592&dopt=citation | en_US |
dc.description.abstract | Genetic compositions of 35 recombinant cold-adapted influenza A(H3N2 and H1N1) candidate live attenuated vaccine strains have been determined. The viruses, which had been obtained by recombination (reassortment) at 25[deg] between contemporary epidemic wild-type strains and cold-adapted A/Ann Arbor/6/60(H2N2), followed by selection for growth at 25[deg] of virus with wild-type HA and NA, have a highly restricted genetic composition. Eighteen of the thirty-five recombinants had RNAs coding for the three polymerase (P) proteins, NP, M, and NS, from the cold-adapted mutant A/Ann Arbor/6/60 had only the HA and NA of the wild-type strains. Only 4 out of 64 theoretically possible combinations of genes coding for nonglycoprotein viral products were detected. The restricted genetic composition of cold-adapted recombinants produced at 25[deg] supports the evaluation of this method of preparing live vaccine strains to determine whether recombinants with constant gene composition have predictable levels of attenuation for man. | en_US |
dc.format.extent | 432600 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Comparative studies of wild-type and cold-mutant (temperature-sensitive) influenza viruses: Nonrandom reassortment of genes during preparation of live virus vaccine candidates by recombination at 25[deg] between recent H3N2 and H1N1 epidemic strains and cold-adapted A/Ann Arbor/6/60 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationother | World Health Organization Collaborating Center for Influenza, Bureau of Laboratories, Center for Disease Control, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333, USA | en_US |
dc.contributor.affiliationother | World Health Organization Collaborating Center for Influenza, Bureau of Laboratories, Center for Disease Control, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333, USA | en_US |
dc.identifier.pmid | 473592 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23515/1/0000470.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0042-6822(79)90386-6 | en_US |
dc.identifier.source | Virology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.