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Instrumental improvements for rapid, detailed kinetic studies of oxygenase activity

dc.contributor.authorCook, Harold W.en_US
dc.contributor.authorFord, Gordonen_US
dc.contributor.authorLands, William E. M.en_US
dc.date.accessioned2006-04-07T17:33:40Z
dc.date.available2006-04-07T17:33:40Z
dc.date.issued1979-07-15en_US
dc.identifier.citationCook, Harold W., Ford, Gordon, Lands, William E. M. (1979/07/15)."Instrumental improvements for rapid, detailed kinetic studies of oxygenase activity." Analytical Biochemistry 96(2): 341-351. <http://hdl.handle.net/2027.42/23527>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DYTNJ8-128/2/a4a2245a059609cd2b6e28e8befbe2faen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/23527
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=474961&dopt=citationen_US
dc.description.abstractThe characteristics of an electronic differentiator that facilitates continuous monitoring of changes in the oxygen concentration measured by a polarographic oxygen electrode are described. In addition, some properties of the electrode membranes that increase the sensitivity of the oxygen-sensing electrode are also described. These modifications greatly improve the accuracy and sensitivity of the biological oxygen monitor for use in kinetic studies of complex enzyme systems such as soybean lipoxygenase or prostaglandin cyclooxygenase. The electronic differentiator also may be used in conjunction with other ratemonitoring instruments such as spectrophotometers and pH meters for accurate quantitation of rapidly changing rates.en_US
dc.format.extent729048 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleInstrumental improvements for rapid, detailed kinetic studies of oxygenase activityen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid474961en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/23527/1/0000483.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(79)90591-8en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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