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A specific, sensitive method for the determination of hyaluronate

dc.contributor.authorJourdian, George W.en_US
dc.contributor.authorWolfman, Melindaen_US
dc.contributor.authorSarber, Roberten_US
dc.contributor.authorDistler, Jack J.en_US
dc.date.accessioned2006-04-07T17:33:43Z
dc.date.available2006-04-07T17:33:43Z
dc.date.issued1979-07-15en_US
dc.identifier.citationJourdian, George W., Wolfman, Melinda, Sarber, Robert, Distler, Jack (1979/07/15)."A specific, sensitive method for the determination of hyaluronate." Analytical Biochemistry 96(2): 474-480. <http://hdl.handle.net/2027.42/23529>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DYTNJ8-12X/2/a7aff7c4a0610ce702a4f71078db4215en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/23529
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=474972&dopt=citationen_US
dc.description.abstractA sensitive and specific assay for hyaluronate was devised. Hyaluronate contained in biological mixtures was digested with a commercially available microbial hyaluronate lyase. The [beta]-[Delta]4,5-eneglucopyranuronic acid residues contained at the nonreducing termini of the resulting oligosaccharides were oxidized with periodic acid to yield, among other products, formyl pyruvic acid. The latter compound reacted with thiobarbituric acid to yield a chromophore with an absorption maximum at 549 nm. Optimal conditions for quantitative assay of hyaluronate are described.en_US
dc.format.extent586559 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA specific, sensitive method for the determination of hyaluronateen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Internal Medicine, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Rackham Arthritis Research Unit, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Internal Medicine, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Rackham Arthritis Research Unit, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Internal Medicine, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Rackham Arthritis Research Unit, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Internal Medicine, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Rackham Arthritis Research Unit, The University of Michigan Medical School, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid474972en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/23529/1/0000485.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(79)90609-2en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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