Methionine transport in S37 cells. Substrate-dependent function of amino acid transport system A in exchange processes
dc.contributor.author | Matthews, Richard H. | en_US |
dc.contributor.author | Zand, Robert | en_US |
dc.date.accessioned | 2006-04-07T17:34:22Z | |
dc.date.available | 2006-04-07T17:34:22Z | |
dc.date.issued | 1979-06-13 | en_US |
dc.identifier.citation | Matthews, Richard H., Zand, Robert (1979/06/13)."Methionine transport in S37 cells. Substrate-dependent function of amino acid transport system A in exchange processes." Biochimica et Biophysica Acta (BBA) - Biomembranes 554(1): 227-233. <http://hdl.handle.net/2027.42/23550> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1T-47T86RN-G5/2/f77f7d51f2be87e2ac09dbc68d780959 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23550 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=454601&dopt=citation | en_US |
dc.description.abstract | Methionine had been observed to interact with two principal transport systems for amino acids in mammalian cells, the A and L systems. The present study of methionine transport and of exchange processes through system A arose in the course of a study to define the specificity of a transinhibition effect caused by cysteine.Methionine uptake through two transport systems in the S37 cell was confirmed by the occurrence of a biphasic double-reciprocal plot for labeled methionine uptake. Preloading cells with methionine stimulated labeled histidine uptake through both systems A and L. Efflux of labeled methionine from cells was stimulated by histidine in a biphasic manner, so that both systems A and L can be used for exchange when methionine is the intracellular amino acid. Aminocycloheptanecarboxylic acid elicited exchange efflux of labeled methionine only through system L. [alpha]-Aminoisobutyric acid and N-methyl-[alpha]-aminoisobutyric acid both stimulated efflux of labeled N-methyl-[alpha]-aminoisobutyric acid from S37 cells. These findings are interpreted a showing that transport system A is capable of functioning as an exchange system depending upon the identity of intracellular and extracellular substrates available. | en_US |
dc.format.extent | 416451 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Methionine transport in S37 cells. Substrate-dependent function of amino acid transport system A in exchange processes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Biophysics Research Division, Institute of Science and Technology, University of Michigan, Ann Arbor, MI 48105, U.S.A.; Department of Physiological Chemistry, The Ohio State University, Columbus, OH 43210, U.S.A. | en_US |
dc.contributor.affiliationum | Biophysics Research Division, Institute of Science and Technology, University of Michigan, Ann Arbor, MI 48105, U.S.A.; Department of Physiological Chemistry, The Ohio State University, Columbus, OH 43210, U.S.A. | en_US |
dc.identifier.pmid | 454601 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23550/1/0000509.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2736(79)90020-8 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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