Enzymic effects of [beta]-glucosidase destruction in mice changes in glucuronidase levels
dc.contributor.author | Hara, Atsushi | en_US |
dc.contributor.author | Radin, Norman S. | en_US |
dc.date.accessioned | 2006-04-07T17:37:25Z | |
dc.date.available | 2006-04-07T17:37:25Z | |
dc.date.issued | 1979-02-01 | en_US |
dc.identifier.citation | Hara, Atsushi, Radin, Norman S. (1979/02/01)."Enzymic effects of [beta]-glucosidase destruction in mice changes in glucuronidase levels." Biochimica et Biophysica Acta (BBA) - General Subjects 582(3): 423-433. <http://hdl.handle.net/2027.42/23645> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1W-47NVPWD-HT/2/bfb9f9d3c13cc118a6fec243cf68f5ff | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23645 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=217440&dopt=citation | en_US |
dc.description.abstract | 1. 1. The injection into mice of a single dose of conduritol B epoxide, a covalent inhibitor of glucosidases, quickly produced changes in tissue levels of [beta]--glucoronidase (EC 3.2.1.31). The specific activity of the enzyme decreased in liver, spleen and kidney while brain showed little change. The inhibitor did not act on glucuronidase in vitro, so the effect of the inhibitor is complex, possibly a result of the loss of glucosidase activity. Since glucuronidase contains glucose, we suggest that the transport of the enzyme between subcellular regions and tissues involves loss of part of the glucose moieties.2. 2. Levels of glucocerebrosidase ( glucohydrolase, EC 3.2.1.45) dropped very rapidly after epoxide injection, reaching a minimum at 1 h in liver. There was a noticeable restoration of activity within the next 1-2 h. Aryl [beta]-glucosidase (EC 3.2.1.21) decrease somewhat less than cerebrosidase, reaching a minimum within 2 h. It too showed some recovery of activity within 3 h.3. 3. Acid phosphatase rose slightly in liver but not in brain. [alpha]--Fucosidase antiotensin-converting enzyme were not affected by the epoxide injection. The latter two enzymes are known to contain glucose.4. 4. Injection of a hemolyzing agent, phenylhydrazine, produced an increased level of glucuronidase in liver and spleen within 6 days, but not in kidney. This enhancement was a little less in mice previously injected with the glucosidase inhibitor.5. 5. Mice injected with the epoxide once a day eight times showed a distinct rise in brain glucuronidase level, as well as a rise in brain weight. However, the other organs showed only the same decrease in glucuronidase specific activity noted with the single injection protocol. It is suggested that the difference is due to the blood-brain barrier, which could slow the loss of brain glucuromidase from the extracellular fluid. | en_US |
dc.format.extent | 700012 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Enzymic effects of [beta]-glucosidase destruction in mice changes in glucuronidase levels | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute (Department of Psychiatry) and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute (Department of Psychiatry) and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 217440 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23645/1/0000609.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0304-4165(79)90134-X | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.