Biosynthesis of keratan sulfate: Purification and properties of a galactosyltransferase from bovine cornea
dc.contributor.author | Christner, James E. | en_US |
dc.contributor.author | Distler, Jack J. | en_US |
dc.contributor.author | Jourdian, George W. | en_US |
dc.date.accessioned | 2006-04-07T17:37:30Z | |
dc.date.available | 2006-04-07T17:37:30Z | |
dc.date.issued | 1979-02 | en_US |
dc.identifier.citation | Christner, James E., Distler, Jack J., Jourdian, George W. (1979/02)."Biosynthesis of keratan sulfate: Purification and properties of a galactosyltransferase from bovine cornea." Archives of Biochemistry and Biophysics 192(2): 548-558. <http://hdl.handle.net/2027.42/23647> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DW2MT0-2FH/2/80ab8ac212318941f9b9918497702a9d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23647 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=35107&dopt=citation | en_US |
dc.description.abstract | A soluble galactosyltransferase was purified 22,000-fold from bovine cornea. The enzyme catalyzes the transfer of galactose from UDP-galactose to N-acetyl--glucosamine, [alpha]- and [beta]-glucosaminides, bovine cornea and nasal septum agalactokeratan, and to glycoproteins containing terminal nonreducing N-acetylglucosaminyl units. When N-acetyl--glucosamine served as acceptor, the product formed by the cornea transferase contained galactose glycosidically linked to carbon atom 4 of N-acetyl--glucosamine; the same glycosidic linkage was found in [14C]keratan preparations isolated from reaction mixtures where keratan containing terminal nonreducing N-acetylglucosaminyl units served as acceptor. The cornea enzyme exhibited a markedly lower Km with keratan than with N-acetyl--glucosamine. The physical and kinetic properties of the cornea galactosyltransferase and of the milk A-protein (A-protein + [alpha]-lactalbumin = lactose synthase), including modulations of acceptor specificity by [alpha]-lactalbumin, were compared. The results of these studies strongly suggest that the two glycosyltransferases are similar, if not identical. Efforts to demonstrate the presence of other soluble galactosyltransferases in cornea were unsuccessful; no change in the ratios of products formed with several acceptors was observed at any stage of purification. It is suggested that in bovine tissues a single galactosyltransferase participates in the synthesis of both high and low molecular weight galactosides including the assembly of the repeating disaccharide [O-[beta]-galactopyranosyl-(1 --> 4)-N-acetylglucosamine] of cornea keratan sulfate. | en_US |
dc.format.extent | 1203450 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Biosynthesis of keratan sulfate: Purification and properties of a galactosyltransferase from bovine cornea | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Rackham Arthritis Research Unit and the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Rackham Arthritis Research Unit and the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Rackham Arthritis Research Unit and the Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.identifier.pmid | 35107 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23647/1/0000611.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(79)90125-5 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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