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Functional assays for mRNA detect many new messages after male meiosis in mice

dc.contributor.authorFujimoto, Hirokazuen_US
dc.contributor.authorErickson, Robert P.en_US
dc.date.accessioned2006-04-07T17:47:05Z
dc.date.available2006-04-07T17:47:05Z
dc.date.issued1982-10-29en_US
dc.identifier.citationFujimoto, H., Erickson, R.P. (1982/10/29)."Functional assays for mRNA detect many new messages after male meiosis in mice." Biochemical and Biophysical Research Communications 108(4): 1369-1375. <http://hdl.handle.net/2027.42/23827>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WBK-4HM8G70-1/2/b749ce48548c181b46e62af1afd60f45en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/23827
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6129844&dopt=citationen_US
dc.description.abstractWe have investigated the changes occurring in the pattern of translatable mRNA species in germ cells during spermatogenesis of mice. Highly homogeneous cell populations of spermatocytes or spermatids were purified. Poly(A)+ mRNA was isolated from each cell population by oligo(dT)-cellulose chromatography. A comparison of meiotic and post-meiotic mRNAs was made by two-dimensional gel analyses of their in vitro synthesized translation products. Among spots identified in the fluorograms of two-dimensional gels, a number of qualitatively new proteins appeared after meiosis. The results suggest that some of poly(A)+ mRNAs are transcribed post-meiotically in haploid germ cells.en_US
dc.format.extent1038076 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleFunctional assays for mRNA detect many new messages after male meiosis in miceen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Human Genetics, Box 015 The University of Michigan Medical School Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Human Genetics, Box 015 The University of Michigan Medical School Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid6129844en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/23827/1/0000066.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/S0006-291X(82)80057-0en_US
dc.identifier.sourceBiochemical and Biophysical Research Communicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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