Functional assays for mRNA detect many new messages after male meiosis in mice
dc.contributor.author | Fujimoto, Hirokazu | en_US |
dc.contributor.author | Erickson, Robert P. | en_US |
dc.date.accessioned | 2006-04-07T17:47:05Z | |
dc.date.available | 2006-04-07T17:47:05Z | |
dc.date.issued | 1982-10-29 | en_US |
dc.identifier.citation | Fujimoto, H., Erickson, R.P. (1982/10/29)."Functional assays for mRNA detect many new messages after male meiosis in mice." Biochemical and Biophysical Research Communications 108(4): 1369-1375. <http://hdl.handle.net/2027.42/23827> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4HM8G70-1/2/b749ce48548c181b46e62af1afd60f45 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23827 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6129844&dopt=citation | en_US |
dc.description.abstract | We have investigated the changes occurring in the pattern of translatable mRNA species in germ cells during spermatogenesis of mice. Highly homogeneous cell populations of spermatocytes or spermatids were purified. Poly(A)+ mRNA was isolated from each cell population by oligo(dT)-cellulose chromatography. A comparison of meiotic and post-meiotic mRNAs was made by two-dimensional gel analyses of their in vitro synthesized translation products. Among spots identified in the fluorograms of two-dimensional gels, a number of qualitatively new proteins appeared after meiosis. The results suggest that some of poly(A)+ mRNAs are transcribed post-meiotically in haploid germ cells. | en_US |
dc.format.extent | 1038076 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Functional assays for mRNA detect many new messages after male meiosis in mice | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Box 015 The University of Michigan Medical School Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Human Genetics, Box 015 The University of Michigan Medical School Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 6129844 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23827/1/0000066.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0006-291X(82)80057-0 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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