A reagent for covalently attaching biotin to proteins via a cleavable connector arm
dc.contributor.author | Mouton, Catharine A. | en_US |
dc.contributor.author | Pang, Dennis T. | en_US |
dc.contributor.author | Natraj, C. V. | en_US |
dc.contributor.author | Shafer, Jules A. | en_US |
dc.date.accessioned | 2006-04-07T17:47:37Z | |
dc.date.available | 2006-04-07T17:47:37Z | |
dc.date.issued | 1982-10-01 | en_US |
dc.identifier.citation | Mouton, Catharine A., Pang, Dennis, Natraj, C. V., Shafer, Jules A. (1982/10/01)."A reagent for covalently attaching biotin to proteins via a cleavable connector arm." Archives of Biochemistry and Biophysics 218(1): 101-108. <http://hdl.handle.net/2027.42/23844> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DN44N8-KF/2/2e108be851608b9d0f73be4ab22c89e5 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23844 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6756312&dopt=citation | en_US |
dc.description.abstract | The preparation of a reagent which can be used to attach biotin to proteins via a cleavable connector arm is described. The reagent, 3-(4-(N-biotinoyl-6-aminocaproyloxy)phenyl)propionic acid N-hydroxysuccinimide ester (BPE), can acylate amino groups of proteins and other compounds via reaction of the N-hydroxysuccinimide ester function. Compounds which have been covalently labeled with BPE can be selectively retrieved (at physiological pH values) from complex mixtures by adsorbing the target compounds on immobilized avidin. These compounds can be released from the avidin by cleavage of the phenyl ester linkage of the connector arm under conditions which don't denature proteins. Insulin was labeled with BPE. The binding of the labeled insulin to immobilized avidin, and its subsequent release by treatment at room temperature with 1 hydroxylamine at pH 7 was demonstrated. | en_US |
dc.format.extent | 782501 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A reagent for covalently attaching biotin to proteins via a cleavable connector arm | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical School, Box 034, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical School, Box 034, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical School, Box 034, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical School, Box 034, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.identifier.pmid | 6756312 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23844/1/0000083.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(82)90325-3 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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