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Purification of human C5a des arg by immunoadsorbent and molecular sieve chromatography
dc.contributor.authorManderino, George L.en_US
dc.contributor.authorSuarez, Adoracion F.en_US
dc.contributor.authorKunkel, Steven L.en_US
dc.contributor.authorWard, Peter A.en_US
dc.contributor.authorHirata, Arthur A.en_US
dc.contributor.authorShowell, Henryen_US
dc.date.accessioned2006-04-07T17:49:09Z
dc.date.available2006-04-07T17:49:09Z
dc.date.issued1982-08-27en_US
dc.identifier.citationManderino, George L., Suarez, Adoracion F., Kunkel, Steven L., Ward, Peter A., Hirata, Arthur A., Showell, Henry (1982/08/27)."Purification of human C5a des arg by immunoadsorbent and molecular sieve chromatography." Journal of Immunological Methods 53(1): 41-50. <http://hdl.handle.net/2027.42/23892>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T2Y-476M00P-31W/2/86af808a7316b8ab0ecaf87d81161f69en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/23892
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6752282&dopt=citationen_US
dc.description.abstractHuman C5a des arg was isolated from complement-activated serum by immunoadsorption followed by Sephadex G-75 chromatography. C5a des arg obtained by this 2-step procedure was shown to be immunologically identical to C5a des arg purified by a conventional multi-step method, homogeneous on SDS-polyacrylamide gels, and biologically active. Although this technique yields approximately the same amount of C5a des arg/liter of activated serum as that obtained by conventional methods, its simplicity and relative rapidity make it a practical alternative.en_US
dc.format.extent642338 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titlePurification of human C5a des arg by immunoadsorbent and molecular sieve chromatographyen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherLaboratory of Immunology, Abbott Laboratories, North Chicago, IL 60064, USAen_US
dc.contributor.affiliationotherLaboratory of Immunology, Abbott Laboratories, North Chicago, IL 60064, USAen_US
dc.contributor.affiliationotherLaboratory of Immunology, Abbott Laboratories, North Chicago, IL 60064, USAen_US
dc.contributor.affiliationotherDepartment of Immunology and Infectious Diseases, Pfizer Research, Croton, CT 06340, U.S.A.en_US
dc.identifier.pmid6752282en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/23892/1/0000131.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0022-1759(82)90238-1en_US
dc.identifier.sourceJournal of Immunological Methodsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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