GABA and bicuculline actions on mouse spinal cord and cortical neurons in cell culture
dc.contributor.author | Nowak, Linda M. | en_US |
dc.contributor.author | Young, Anne B. | en_US |
dc.contributor.author | Macdonald, Robert L. | en_US |
dc.date.accessioned | 2006-04-07T17:50:08Z | |
dc.date.available | 2006-04-07T17:50:08Z | |
dc.date.issued | 1982-07-22 | en_US |
dc.identifier.citation | Nowak, Linda M., Young, Anne B., Macdonald, Robert L. (1982/07/22)."GABA and bicuculline actions on mouse spinal cord and cortical neurons in cell culture." Brain Research 244(1): 155-164. <http://hdl.handle.net/2027.42/23922> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYR-4847S39-141/2/fcaaa4dfd3482e1649a5d365903b7821 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23922 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6288177&dopt=citation | en_US |
dc.description.abstract | The neutral amino acid [gamma]-aminobutyric acid (GABA) produced membrane hyperpolarization and increased membrane chloride ion conductance of spinal cord (SC) and cortical (CTX) neurons in cell culture. GABA dose-response curves were obtained for SC neurons by pressure applying known concentrations of GABA from micropipettes with large tips (miniperfusion pipettes). GABA response threshold was about 2 [mu]M and large responses were elicited at GABA concentrations greater than 10 [mu]M. Bicuculline (BICUC) (0.1-10 [mu]M) reversibly antagonized GABA responses on both SC and CTX neurons with a half maximal inhibitory concentration of about 1 [mu]M. BICUC antagonism of GABA responses was competitive (Lineweaver-Burke analysis). These results are compared with data on GABA and BICUC displacement of [3H]GABA binding to membranes of SC and CTX neurons in cell culture. It is suggested that high affinity GABA receptors are likely to be relevant for postsynaptic GABA responses while low affinity GABA receptors may be presynaptic. | en_US |
dc.format.extent | 925390 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | GABA and bicuculline actions on mouse spinal cord and cortical neurons in cell culture | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neurosciences Program and (A.B.Y. and R.L.M.) Department of Neurology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Neurosciences Program and (A.B.Y. and R.L.M.) Department of Neurology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Neurosciences Program and (A.B.Y. and R.L.M.) Department of Neurology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 6288177 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23922/1/0000167.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-8993(82)90913-1 | en_US |
dc.identifier.source | Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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