Monoclonal antibodies to human sperm antigens
dc.contributor.author | Chi-Yu Gregory Lee, | en_US |
dc.contributor.author | Huang, Yuan-Shen | en_US |
dc.contributor.author | Huang, Chien-Hwai | en_US |
dc.contributor.author | Ping-C. Hu, | en_US |
dc.contributor.author | Menge, Alan C. | en_US |
dc.date.accessioned | 2006-04-07T17:50:31Z | |
dc.date.available | 2006-04-07T17:50:31Z | |
dc.date.issued | 1982-07 | en_US |
dc.identifier.citation | Chi-Yu Gregory Lee, , Huang, Yuan-Shen, Huang, Chien-Hwai, Ping-C Hu, , Menge, Alan C. (1982/07)."Monoclonal antibodies to human sperm antigens." Journal of Reproductive Immunology 4(3): 173-181. <http://hdl.handle.net/2027.42/23934> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T8W-476M2KY-6T/2/6ab990d67e7e29e4519f20f06de46de5 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23934 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7120206&dopt=citation | en_US |
dc.description.abstract | To elucidate the molecular nature of human sperm autoantigens, attempts were made to raise monoclonal antibodies against these antigens by hybridoma techniques. After successive immunizations with the particulate fractions of human sperm extract in BALB/c mice, the spleen cells were fused with P3-X63-Ag8 myeloma cells. Several clones and their subclones were obtained and shown by microplate radioimmunoassay to produce antibodies against human sperm antigens. When SDS gel/protein blot radioimmunobinding was used for further molecular analysis, three independently derived clones were shown to produce antibodies, all of which cross-reacted with the same two human sperm antigens with a molecular weight of about 10 000. Using an indirect immunofluorescence assay, antibodies produced by these clones were shown to react with antigens localized on the acrosomal regions of human spermatozoa. Monoclonal antibodies produced by other clones, however, showed no cross-reactivity with any of the blotted proteins from SDS gels of human spermatozoa. Some possible reasons for this are presented. | en_US |
dc.format.extent | 594134 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Monoclonal antibodies to human sperm antigens | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Obstetrics and Gynecology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Obstetrics and Gynecology, University of Michigan Medical Center, Ann Arbor, Mich., U.S.A. | en_US |
dc.contributor.affiliationother | Department of Obstetrics and Gynaecology, University of British Columbia, Vancouver, Canada | en_US |
dc.contributor.affiliationother | Cancer Research Center, University of North Carolina, Chapel Hill, N.C., U.S.A. | en_US |
dc.contributor.affiliationother | Cancer Research Center, University of North Carolina, Chapel Hill, N.C., U.S.A. | en_US |
dc.contributor.affiliationother | Cancer Research Center, University of North Carolina, Chapel Hill, N.C., U.S.A. | en_US |
dc.identifier.pmid | 7120206 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23934/1/0000180.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0165-0378(82)90035-3 | en_US |
dc.identifier.source | Journal of Reproductive Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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