Cold-adapted recombinants of influenza a virus in MDCK cells I. Development and characterization of A/Ann Arbor/6/60 x A/Alaska /6/77 recombinant viruses
dc.contributor.author | Odagiri, Takato | en_US |
dc.contributor.author | DeBorde, Dan C. | en_US |
dc.contributor.author | Maassab, Hunein F. | en_US |
dc.date.accessioned | 2006-04-07T17:52:15Z | |
dc.date.available | 2006-04-07T17:52:15Z | |
dc.date.issued | 1982-05 | en_US |
dc.identifier.citation | Odagiri, Takato, DeBorde, Dan C., Maassab, Hunein F. (1982/05)."Cold-adapted recombinants of influenza a virus in MDCK cells I. Development and characterization of A/Ann Arbor/6/60 x A/Alaska /6/77 recombinant viruses." Virology 119(1): 82-95. <http://hdl.handle.net/2027.42/23988> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WXR-4BMSS09-9F/2/671daef9b82c8cb2d650bf318ef544ac | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/23988 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7072155&dopt=citation | en_US |
dc.description.abstract | Recombinant influenza viruses made at 25 and 33[deg] in Madin-Darby canine kidney (MDCK) cells using the cold-adapted A/Ann Arbor/6/60 virus and the wild-type A/Alaska/6/77 virus were biologically and genetically analyzed. Eight recombinants were separated into two phenotypic groups based on cold-adapted (ca) and temperature-sensitive (ts) markers: ca and ts, ca and non-ts. The ca recombinants showed different degrees of cold adaptibility (DOCA) and different patterns of virus growth at 25[deg]. All recombinants contained at most three genes from the cold variant A/Ann Arbor/6/60 virus (triple-gene recombinant) and most contained two or one gene from the cold variant parent (double-gene and single-gene recombinants, respectively). Further, the same three genes, RNA2, RNA3, and RNA5 (NA) were the only ca A/Ann Arbor/6/60 genes found in the various recombinants. Two clones contained all three A/Ann Arbor/6/60 genes and were both cold-adapted (ca) and temperature-sensitive (ts). All other recombinant clones were ca and non-ts, and contained RNA2 and/or RNA5 (NA). Each set of single-gene ca recombinants correlated with a different, but specific cold-adapted characteristic exhibited by their growth curves at 25[deg]. Single-gene recombinants containing only the RNA2 of A/Ann Arbor/6/60 virus showed rapid growth early in infection and intermediate final virus yield (between the titer of virus yield for the ca A/Ann Arbor/6/60 virus and the wild-type A/Alaska/6/77 virus; while the single-gene recombinant containing only the RNA5 (NA) of A/Ann Arbor/6/60 virus showed slow growth early in infection, but a high final virus yield (equivalent to that of the ca A/Ann Arbor/6/60 parent). The double-gene recombinant containing both these genes showed both rapid growth early in infection and a high final virus yield. Thus, cold adaptation can be transferred to recombinant viruses by at least two independent genes each of which can confer the cold-adaptive property by its own pathway. The genetic basis for temperature sensitivity involves both RNA2 and RNA3, but remains ambiguous in the absence of a single-gene recombinant containing only RNA3 of the cold variant. | en_US |
dc.format.extent | 3142904 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Cold-adapted recombinants of influenza a virus in MDCK cells I. Development and characterization of A/Ann Arbor/6/60 x A/Alaska /6/77 recombinant viruses | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Epidemiology, School of Public Health, The University of Michigan, 109 Observatory Street, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Epidemiology, School of Public Health, The University of Michigan, 109 Observatory Street, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Epidemiology, School of Public Health, The University of Michigan, 109 Observatory Street, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 7072155 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/23988/1/0000237.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0042-6822(82)90067-8 | en_US |
dc.identifier.source | Virology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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