Iron-ethylenediaminetetraacetic acid (EDTA)-catalyzed superoxide dismutation revisited: An explanation of why the dismutase activity of Fe-EDTA cannot be detected in the cytochrome c/xanthine oxidase assay system
dc.contributor.author | Bull, C. | en_US |
dc.contributor.author | Fee, James A. | en_US |
dc.contributor.author | O'Neill, P. | en_US |
dc.contributor.author | Fielden, E. M. | en_US |
dc.date.accessioned | 2006-04-07T17:52:42Z | |
dc.date.available | 2006-04-07T17:52:42Z | |
dc.date.issued | 1982-05 | en_US |
dc.identifier.citation | Bull, C., Fee, J. A., O'Neill, P., Fielden, E. M. (1982/05)."Iron-ethylenediaminetetraacetic acid (EDTA)-catalyzed superoxide dismutation revisited: An explanation of why the dismutase activity of Fe-EDTA cannot be detected in the cytochrome c/xanthine oxidase assay system." Archives of Biochemistry and Biophysics 215(2): 551-555. <http://hdl.handle.net/2027.42/24002> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DV0952-1JG/2/a80007709985cbb10b3707920d9b8926 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24002 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6284055&dopt=citation | en_US |
dc.description.abstract | The recent assertion of [1.] that Fe-EDTA does not catalyze superoxide dismutation is disputed. By directly observing superoxide generated during pulse radiolysis, we have confirmed the results of a previous study ( [2.]) which concluded that Fe-EDTA catalyzed superoxide dismutation. We also demonstrate that the reaction of Fe(II)-EDTA, formed during catalyzed superoxide dismutation, with cytochrome c, the probe molecule in the cytochrome c/xanthine oxidase/xanthine assay system for superoxide dismutase activity, is sufficiently rapid ([12.]) to obscure the weak catalysis of superoxide dismutation by Fe-EDTA. | en_US |
dc.format.extent | 392336 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Iron-ethylenediaminetetraacetic acid (EDTA)-catalyzed superoxide dismutation revisited: An explanation of why the dismutase activity of Fe-EDTA cannot be detected in the cytochrome c/xanthine oxidase assay system | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Biophysics Research Division and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Biophysics Research Division and Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Division of Physics, Institute of Cancer Research, Clifton Avenue, Surrey SM2 2PX, United Kingdom | en_US |
dc.contributor.affiliationother | Division of Physics, Institute of Cancer Research, Clifton Avenue, Surrey SM2 2PX, United Kingdom | en_US |
dc.identifier.pmid | 6284055 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24002/1/0000251.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(82)90115-1 | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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