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Enrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brains

dc.contributor.authorBedows, Elliotten_US
dc.contributor.authorKohne, David E.en_US
dc.contributor.authorTourtellotte, Wallace W.en_US
dc.contributor.authorPayne, Francis E.en_US
dc.date.accessioned2006-04-07T17:54:38Z
dc.date.available2006-04-07T17:54:38Z
dc.date.issued1982-02en_US
dc.identifier.citationBedows, Elliott, Kohne, David E., Tourtellotte, Wallace W., Payne, Francis E. (1982/02)."Enrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brains." Journal of Virological Methods 4(1): 37-43. <http://hdl.handle.net/2027.42/24061>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T96-476W86X-1P/2/1d33dbb6075fd6b740341d41b4f5842fen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/24061
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6895898&dopt=citationen_US
dc.description.abstractA procedure has been developed which facilitates the detection of measles virus RNA sequences in human brains. The procedure involves isolating subviral components (nucleocapsids) from brain tissues prior to RNA purification, followed by hybridization of these RNAs to cDNA synthesized from measles virus 50 S RNA template. Using these techniques we were able to obtain an RNA fraction which was manyfold enriched in measles virus-specific RNA, relative to unfractionated subacute sclerosing panencephalitis (SSPE) brain RNAs. 70-100% of the measles virus-specific RNA present in these SSPE brain samples were recovered in this enriched fraction.en_US
dc.format.extent443908 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleEnrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brainsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartments of Epidemiology and Anaesthesiology, School of Public Health and School of Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationumProfessor of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationotherCenter for Neurologic Study, 11575 Sorrento Valley Road, Suite 203, San Diego, CA 92121, U.S.A.en_US
dc.contributor.affiliationotherChief, Neurology Service V.A. Wadsworth Hospital Center, Wilshire and Sawtelle Boulevards, Los Angeles, CA 90073, U.S.A.en_US
dc.identifier.pmid6895898en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/24061/1/0000313.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0166-0934(82)90052-0en_US
dc.identifier.sourceJournal of Virological Methodsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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