Effects of dimethyl sulfoxide on the globally ischemic heart: Possible general relevance to hypothermic organ preservation
dc.contributor.author | Shlafer, Marshal | en_US |
dc.contributor.author | Kane, Paula F. | en_US |
dc.contributor.author | Kirsh, Marvin M. | en_US |
dc.date.accessioned | 2006-04-07T17:54:42Z | |
dc.date.available | 2006-04-07T17:54:42Z | |
dc.date.issued | 1982-02 | en_US |
dc.identifier.citation | Shlafer, Marshal, Kane, Paula F., Kirsh, Marvin M. (1982/02)."Effects of dimethyl sulfoxide on the globally ischemic heart: Possible general relevance to hypothermic organ preservation." Cryobiology 19(1): 61-69. <http://hdl.handle.net/2027.42/24063> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WD5-4F1RW9F-F4/2/f5513ccf30127b39bac4cc795a043af9 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24063 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7039976&dopt=citation | en_US |
dc.description.abstract | Isolated perfused rabbit hearts were made globally ischemic for 2 hr, then reperfused. For 5 min before and after ischemia hearts were perfused with hypothermic (20 or 27 [deg]C), hypoxic, substrate-free cardioplegic solutions, some of which contained 70 mM dimethyl sulfoxide. Postischemic ventricular pressure development, spontaneous heart rate, coronary flow, lactate dehydrogenase release, tissue Ca2+ content, and in vitro mitochondrial oxidative phosphorylation were used to evaluate the protective effects of the various solutions. Aside from the expected observations that cold cardioplegia lessens ischemic damage, we found that dimethyl sulfoxide gave no indication that it exacerbated ischemic damage or lessened the protection afforded by cardioplegia. We also found that, compared to values measured in comparable drug-free treated hearts, dimethyl sulfoxide significantly improved mitochondrial State 3 respiratory rates, respiratory control, and oxidative phosphorylation rates, and essentially prevented mitochondrial changes due to ischemia and reperfusion. We propose that dimethyl sulfoxide may act as a "scavenger" of cytotoxic free radicals, many of which are known to be generated by mitochondria during reoxygenation. Since hypoxia, ischemia, and reoxygenation are common accompaniments of most organ preservation protocols, we suggest that low concentrations of dimethyl sulfoxide might serve as a useful adjunct to organ preservation in the nonfrozen state, when cryoprotective concentrations are not needed. | en_US |
dc.format.extent | 784794 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Effects of dimethyl sulfoxide on the globally ischemic heart: Possible general relevance to hypothermic organ preservation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Pharmacology and Surgery, Section of Thoracic Surgery, The University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Pharmacology and Surgery, Section of Thoracic Surgery, The University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Pharmacology and Surgery, Section of Thoracic Surgery, The University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.identifier.pmid | 7039976 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24063/1/0000315.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0011-2240(82)90125-0 | en_US |
dc.identifier.source | Cryobiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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