Cloning of mouse [beta]-casein gene sequences
dc.contributor.author | Mehta, Nozer M. | en_US |
dc.contributor.author | El-Gewely, M. Raafat | en_US |
dc.contributor.author | Joshi, Jay | en_US |
dc.contributor.author | Helling, Robert B. | en_US |
dc.contributor.author | Banerjee, Mihir R. | en_US |
dc.date.accessioned | 2006-04-07T18:00:19Z | |
dc.date.available | 2006-04-07T18:00:19Z | |
dc.date.issued | 1981-11 | en_US |
dc.identifier.citation | Mehta, Nozer M., El-Gewely, M. Raafat, Joshi, Jay, Helling, Robert B., Banerjee, Mihir R. (1981/11)."Cloning of mouse [beta]-casein gene sequences." Gene 15(2-3): 285-288. <http://hdl.handle.net/2027.42/24211> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T39-47T1JD7-28/2/ef64fa4707ed2b947c58e8e52de4b1c7 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24211 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7297856&dopt=citation | en_US |
dc.description.abstract | Casein messenger RNAs (mRNAcsn) were purified from lactating mammary glands of BALB/c mice and used as a starting material for cloning of casein gene sequences. Double-stranded casein cDNA (ds-cDNAscn) was prepared and blunt-end ligated to HindIII-specific DNA linker molecules. After digestion with HindIII, the dsDNAcsn was inserted into the HindIII site of plasmid pBR322, using T4 DNA ligase. Escherchia coli strain RH202 was transformed with the hybrid plasmids, and transformants were selected for resistance to ampicillin. Electrophoresis of HindIII-digested hybrid plasmid DNAs, followed by Souther transfer and hybridization to 9[32P]cDNAcsn, revealed that one of the hybrid-plasmid-containing colonies, designated pCas51, contained a 400-bp insert which hybridized to the [32P]cDNAcsn. Purification of the individual casein mRNAs (mRNAcsn [alpha], [beta] and [gamma]) and solution hybridization of nick-translated insert DNA to each of these revealed that pCas51 contained sequences complementary primarily to mRNAcsn [beta]. | en_US |
dc.format.extent | 700813 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Cloning of mouse [beta]-casein gene sequences | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Biological Sciences, University of Michigan, Natural Science Building, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Division of Biological Sciences, University of Michigan, Natural Science Building, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Tumor Biology Laboratory, School of Life Sciences, University of Nebraska, 201 Lyman Hall, Lincoln, NE 68588, USA | en_US |
dc.contributor.affiliationother | Tumor Biology Laboratory, School of Life Sciences, University of Nebraska, 201 Lyman Hall, Lincoln, NE 68588, USA | en_US |
dc.contributor.affiliationother | Tumor Biology Laboratory, School of Life Sciences, University of Nebraska, 201 Lyman Hall, Lincoln, NE 68588, USA | en_US |
dc.identifier.pmid | 7297856 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24211/1/0000470.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-1119(81)90138-4 | en_US |
dc.identifier.source | Gene | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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