Developmental program of PGK-1 and PGK-2 isozymes in spermatogenic cells of the mouse: Specific activities and rates of synthesis
dc.contributor.author | Kramer, James M. | en_US |
dc.contributor.author | Erickson, Robert P. | en_US |
dc.date.accessioned | 2006-04-07T18:01:01Z | |
dc.date.available | 2006-04-07T18:01:01Z | |
dc.date.issued | 1981-10-15 | en_US |
dc.identifier.citation | Kramer, James M., Erickson, Robert P. (1981/10/15)."Developmental program of PGK-1 and PGK-2 isozymes in spermatogenic cells of the mouse: Specific activities and rates of synthesis." Developmental Biology 87(1): 37-45. <http://hdl.handle.net/2027.42/24231> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WDG-4DMW7J1-1N/2/7104c2b30e8fbdede1840f84473bd0d3 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24231 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7286419&dopt=citation | en_US |
dc.description.abstract | The specific activities and synthesis of the ubiquitous isozyme, PGK-1, and the testis-specific isozyme, PGK-2, have been quantitated and localized in spermatogenic cells of the mouse. There is a fivefold increase in total PGK specific activity between immature and adult testes which begins at approximately 30 days of age, coincident with the appearance of late-middle stage spermatids. The increase in total PGK is entirely due to the appearance and increase of the PGK-2 isozyme. Rates of PGK synthesis were measured by labeling testicular cells in vitro with [3H]leucine and purifying the PGK isozymes. When total testicular cells were examined, PGK-2 synthesis was detectable after 22 days of age at very low levels and increased in older testes to a level of 0.5% of total protein synthesis. PGK-1 synthesis remained relatively constant at all ages at a level 100-fold lower (0.005%). Testicular cells were separated into highly enriched fractions of particular spermatogenic stages by centrifugal elutriation. The PGK-1 synthesis rates were, again, very low and not significantly different between the various spermatogenic stages. PGK-2 synthesis was low to nondetectable in pachytene spermatocytes, increased to 0.07% in early spermatids and represented 0.7% of total protein synthesis in late spermatids. This increased rate of PGK-2 synthesis appears to require an increase in the amount of PGK-2 mRNA in late spermatids, cells in which no active RNA synthesis is detectable. | en_US |
dc.format.extent | 1140316 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Developmental program of PGK-1 and PGK-2 isozymes in spermatogenic cells of the mouse: Specific activities and rates of synthesis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 7286419 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24231/1/0000491.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0012-1606(81)90058-0 | en_US |
dc.identifier.source | Developmental Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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