Activation of CTP : Phosphocholine cytidylyltransferase in rat lung by fatty acids
dc.contributor.author | Feldman, Douglas A. | en_US |
dc.contributor.author | Brubaker, Pamela G. | en_US |
dc.contributor.author | Weinhold, Paul A. | en_US |
dc.date.accessioned | 2006-04-07T18:03:36Z | |
dc.date.available | 2006-04-07T18:03:36Z | |
dc.date.issued | 1981-07-24 | en_US |
dc.identifier.citation | Feldman, Douglas A., Brubaker, Pamela G., Weinhold, Paul A. (1981/07/24)."Activation of CTP : Phosphocholine cytidylyltransferase in rat lung by fatty acids." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 665(1): 53-59. <http://hdl.handle.net/2027.42/24302> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1X-47G2PHW-6V/2/3561d8b88ea3142c243d99a6e21c90b2 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24302 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6269641&dopt=citation | en_US |
dc.description.abstract | CTP : phosphocholine cytidylyltransferase activity exists in both the microsome and cytosol fractions of adult lung, 36 and 59%, respectively. Although these enzyme activities are stimulated in vitro by added lipid activators (i.e. phosphatidylglycerol), there are significant levels of activity in the absence of added lipid. We have removed endogenous lipid material from microsome and cytosol preparations of rat lung by rapid extraction with isopropyl ether. The extraction procedure did not cause any loss of cytidylyltransferase activity in the cytosol. After the extraction the enzyme was almost completely dependent upon added lipid activator. Isopropyl ether extraction of microsome preparations produced a loss of 40% of the cytidylyltransferase activity, when measured in the presence of added phosphatidylglycerol. Lipid material extracted into isopropyl ether restored the cytidylyltransferase activity in cytosol. The predominant species of enzyme activator in the isopropyl ether extracts was fatty acid. A variety of naturally occurring unsaturated fatty acids stimulated the cytidylyltransferase to the same extent as phosphatidylglycerol. Saturated fatty acids were inactive. | en_US |
dc.format.extent | 658248 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Activation of CTP : Phosphocholine cytidylyltransferase in rat lung by fatty acids | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Veterans Administration Medical Center and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48105, U.S.A. | en_US |
dc.contributor.affiliationum | Veterans Administration Medical Center and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48105, U.S.A. | en_US |
dc.contributor.affiliationum | Veterans Administration Medical Center and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48105, U.S.A. | en_US |
dc.identifier.pmid | 6269641 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24302/1/0000568.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2760(81)90231-9 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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