An in vivo-in vitro alkaline DNA unwinding assay for hepatic DNA damage: Comparison with the alkaline sucrose gradient centrifugation technique
dc.contributor.author | Storer, Richard D. | en_US |
dc.contributor.author | Conolly, Rory B. | en_US |
dc.date.accessioned | 2006-04-07T18:18:00Z | |
dc.date.available | 2006-04-07T18:18:00Z | |
dc.date.issued | 1984-11-01 | en_US |
dc.identifier.citation | Storer, Richard D., Conolly, Rory B. (1984/11/01)."An in vivo-in vitro alkaline DNA unwinding assay for hepatic DNA damage: Comparison with the alkaline sucrose gradient centrifugation technique." Analytical Biochemistry 142(2): 351-359. <http://hdl.handle.net/2027.42/24653> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DYN47Y-53/2/5b9a38d516236f87c6a05a335e1256b0 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/24653 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6528974&dopt=citation | en_US |
dc.description.abstract | An in vivo-in vitro alkaline DNA unwinding assay for single-strand breaks and/or alkalilabile lesions in hepatic DNA is described. The assay involves isolation of hepatic nuclei from mice, alkaline denaturation and unwinding of hepatic DNA, separation of single- and double-stranded DNA by hydroxylapatite batch chromatography, and quantitation of DNA in chromatographic fractions by a fluorometric assay. The method allows for the sensitive detection of hepatic DNA damage following in vivo administration of xenobiotics. Using this procedure, DNA fragmentation was demonstrated in alkali after administration of as little as 0.5 mg/kg of N-nitrosodimethylamine (DMN) to male B6C3F1 mice. A comparison of this technique with a similar alkaline sucrose density gradient centrifugation assay demonstrated comparable limits of sensitivity for the two procedures. | en_US |
dc.format.extent | 817961 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | An in vivo-in vitro alkaline DNA unwinding assay for hepatic DNA damage: Comparison with the alkaline sucrose gradient centrifugation technique | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Toxicology Research Laboratory, Department of Environmental and Industrial Health, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109-2029, USA | en_US |
dc.contributor.affiliationum | Toxicology Research Laboratory, Department of Environmental and Industrial Health, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48109-2029, USA | en_US |
dc.identifier.pmid | 6528974 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/24653/1/0000066.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(84)90476-7 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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